Characterization of the Human Interleukin-2 Receptor β-Chain Gene Promoter: Regulation of Promoter Activity by ets Gene Products
| Autor: | J X, Lin, N K, Bhat, S, John, W S, Queale, W J, Leonard |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Binding Sites
Base Sequence Proto-Oncogene Proteins c-ets Molecular Sequence Data Receptors Interleukin-2 DNA Cell Biology Proto-Oncogene Protein c-ets-1 Enhancer Elements Genetic Gene Expression Regulation Mutagenesis Organ Specificity Proto-Oncogene Proteins Humans Tetradecanoylphorbol Acetate Amino Acid Sequence Promoter Regions Genetic Molecular Biology HeLa Cells Transcription Factors Research Article |
| Zdroj: | Molecular and Cellular Biology. 13:6201-6210 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.13.10.6201-6210.1993 |
| Popis: | The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|