Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes
| Autor: | Chika Higuchi, Kei Miyamoto, Kazuo Yamagata, Haruka Ikegami, Yoshihiko Hosoi, Kazuya Matsumoto, Kouhei Nagai, Masayuki Anzai, Kohtaro Morita, Hiromi Kato, Tasuku Mitani, Yoshitomo Taguchi, Yuki Hatanaka, Mikiko Tokoro |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Proteomics 0301 basic medicine Mouse Zygote Hydrogen peroxide (H2O2) Active Transport Cell Nucleus Endogeny Fertilization in Vitro Peroxiredoxin 1 medicine.disease_cause Zygotes Epigenesis Genetic 03 medical and health sciences 0302 clinical medicine medicine Animals Cells Cultured Cellular localization Cell Nucleus chemistry.chemical_classification Mice Inbred ICR Reactive oxygen species Cumulus Cells Microscopy Confocal 030219 obstetrics & reproductive medicine Chemistry Peroxiredoxin (PRDX) Hydrogen Peroxide Peroxiredoxins DNA Methylation Oxidants Cell biology Oxidative Stress 030104 developmental biology 5-Methylcytosine Original Article Ectogenesis Female Animal Science and Zoology Reactive Oxygen Species Peroxiredoxin Reprogramming Oxidative stress |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| Popis: | Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2O2). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H2O2 enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT. |
| Databáze: | OpenAIRE |
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