D-Alanylation of teichoic acids contributes to Lactobacillus plantarum-mediated Drosophila growth during chronic undernutrition
| Autor: | François Leulier, Hugo Gervais, Gillet B, Renata C. Matos, Pascal Courtin, Sandrine Hughes, Maria Elena Martino, Martin Schwarzer, Dali Ma, Pauline Joncour, Anne-Laure Bulteau, Marie-Pierre Chapot-Chartier |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Fachbereich Chemie der Philipps-Universität Marburg (WZMW), Philipps Universität Marburg = Philipps University of Marburg, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, AGROCAMPUS OUEST, Plateforme nationale de Paléogénétique (Palgene), École normale supérieure de Lyon (ENS de Lyon), Fondation pour la Recherche Médicale [SPF20140129318], European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [N8659510], FINOVI foundation, EMBO Young Investigator Program, European Project: 309704,EC:FP7:ERC,ERC-2012-StG_20111109,MUTFLYGUTBACT(2013) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Operon 030106 microbiology Immunology Peptidoglycan Applied Microbiology and Biotechnology Microbiology Bacterial cell structure Bacterial genetics Cell wall 03 medical and health sciences chemistry.chemical_compound Cell Wall Genetics Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Symbiosis Biological Phenomena Teichoic acid Alanine biology Microbiota Malnutrition fungi Bacterial Cell Biology biology.organism_classification Drosophila Genes Bacterial Lactobacillus plantarum Larva Mutagenesis Teichoic Acids [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology carbohydrates (lipids) Genes chemistry bacteria Bacteria |
| Zdroj: | Nature Microbiology Nature Microbiology, 2017, 2 (12), pp.1635-1647. ⟨10.1038/s41564-017-0038-x⟩ |
| ISSN: | 2058-5276 |
| DOI: | 10.1038/s41564-017-0038-x⟩ |
| Popis: | The microbial environment influences animal physiology. However, the underlying molecular mechanisms of such functional interactions are largely undefined. Previously, we showed that during chronic undernutrition, strains of Lactobacillus plantarum, a major commensal partner of Drosophila, promote host juvenile growth and maturation partly through enhanced expression of intestinal peptidases. By screening a transposon insertion library of Lactobacillus plantarum in gnotobiotic Drosophila larvae, we identify a bacterial cell-wall-modifying machinery encoded by the pbpX2-dlt operon that is critical to enhance host digestive capabilities and promote animal growth and maturation. Deletion of this operon leads to bacterial cell wall alteration with a complete loss of d-alanylation of teichoic acids. We show that L. plantarum cell walls bearing d-alanylated teichoic acids are directly sensed by Drosophila enterocytes to ensure optimal intestinal peptidase expression and activity, juvenile growth and maturation during chronic undernutrition. We thus conclude that besides peptidoglycan, teichoic acid modifications participate in the host–commensal bacteria molecular dialogue occurring in the intestine. Under nutritional limitation, modification of the Lactobacillus plantarum cell wall by d-alanylation of teichoic acids is important for host intestinal peptidase expression and consequently growth of the Drosophila host, providing further insights into host–commensal interactions. |
| Databáze: | OpenAIRE |
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