Endothelin-1 inhibits human osteoblastic cell differentiation: Influence of connexin-43 expression level

Autor: Corinne Niger, Marc Mesnil, Graziello Geneau, Laurent Cronier, Céline Fiorini, Norah Defamie, Georges Pointis
Přispěvatelé: Department of Orthopaedics, Institut de physiologie et biologie cellulaires (IPBC), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Connexines et prolifération germinale : Physiopathologie cellulaire et moléculaire, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Ligue Nationale contre le Cancer
Rok vydání: 2007
Předmět:
connexin-43
Connexin
Biochemistry
Cell Line
Extracellular matrix
03 medical and health sciences
gap junctional communication
0302 clinical medicine
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Molecular Biology
Cell Proliferation
030304 developmental biology
0303 health sciences
Osteoblasts
Endothelin-1
biology
Gap Junctions
Cell Differentiation
Cell Biology
Receptor
Endothelin A
Endothelin 1
Phenotype
Cell biology
Crosstalk (biology)
Connexin 43
osteoblast
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Osteocalcin
biology.protein
Alkaline phosphatase
endothelin
differentiation process
030217 neurology & neurosurgery
Intracellular
Zdroj: Journal of Cellular Biochemistry
Journal of Cellular Biochemistry, Wiley, 2008, 103, pp.110-122. ⟨10.1002/jcb.21390⟩
ISSN: 1097-4644
0730-2312
DOI: 10.1002/jcb.21390
Popis: (IF : 3,54); International audience; Gap junctional intercellular communication (GJIC) permits coordinated cellular activities during developmental and differentiation processes. In bone, the involvement of the gap junctional protein, connexin-43 (Cx43), and of GJIC in osteoblastic differentiation and mineralization of the extracellular matrix has been previously demonstrated. Former studies have shown that endothelin-1 (ET-1) was also implicated in the control of osteoblastic proliferation and differentiation. However, depending on the cellular models, ET-1 has been shown to decrease or increase osteoblastic differentiation markers. As no data were available on the ET-1 effect on GJIC and Cx43 expression in osteoblastic cells, we analyzed here the possible crosstalk between Cx43 and ET-1 in a human cell line (hFOB 1.19) which displays different Cx43 expression levels and phenotypes when cultured at 33.5 or 39 degrees C. The presence of ET-1 (10(-8) M) for 2-12 days of culture did not significantly alter the proliferation rate of hFOB cells whatever their phenotype. In contrast, ET-1 induced a differential inhibitory effect on the biochemical differentiation markers (alkaline phosphatase activity and osteocalcin expression) with a significant reduction in the differentiated phenotype at 39 degrees C, whereas no effects were measured at 33.5 degrees C. The inhibitory effect was linked to a decrease of GJIC and of Cx43 both at transcriptional and protein levels. Altogether, our results suggest that Cx43 expression level could influence the action of ET-1 on human osteoblastic cell differentiation. Our data also indicate that the gap junctional protein could play a pivotal role in the response of osteoblasts to mitogenic factors implicated in bone pathologies.
Databáze: OpenAIRE
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