Plasmonic nanoparticle amyloid corona for screening Aβ oligomeric aggregate-degrading drugs
| Autor: | Dongsung Park, Kyo Seon Hwang, Sang Won Lee, Jeong Hoon Lee, Wonseok Lee, Insu Kim, Dae Sung Yoon, Dongtak Lee, Gyudo Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Drug
Amyloid Proteases Time Factors Science media_common.quotation_subject Protease XIV Metal Nanoparticles General Physics and Astronomy Nanoparticle Protein aggregation Ligands Oligomer Article General Biochemistry Genetics and Molecular Biology Protein Aggregates chemistry.chemical_compound mental disorders media_common Amyloid beta-Peptides Multidisciplinary Drug discovery General Chemistry Kinetics Drug screening chemistry Biophysics Nanoparticles Gold Biomedical engineering |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-20611-4 |
| Popis: | The generation of toxic amyloid β (Aβ) oligomers is a central feature of the onset and progression of Alzheimer’s disease (AD). Drug discoveries for Aβ oligomer degradation have been hampered by the difficulty of Aβ oligomer purification and a lack of screening tools. Here, we report a plasmonic nanoparticle amyloid corona (PNAC) for quantifying the efficacy of Aβ oligomeric aggregate-degrading drugs. Our strategy is to monitor the drug-induced degradation of oligomeric aggregates by analyzing the colorimetric responses of PNACs. To test our strategy, we use Aβ-degrading proteases (protease XIV and MMP-9) and subsequently various small-molecule substances that have shown benefits in the treatment of AD. We demonstrate that this strategy with PNAC can identify effective drugs for eliminating oligomeric aggregates. Thus, this approach presents an appealing opportunity to reduce attrition problems in drug discovery for AD treatment. Effective screening of drugs to treat amyloid oligomeric aggregates associated with Alzheimer’s is needed for drug development. Here the authors report on the creation of an amyloid corona around a plasmonic nanoparticle to monitor amyloid degradation when exposed to potential drugs and demonstrate application. |
| Databáze: | OpenAIRE |
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