Novel G Protein-Coupled Oestrogen Receptor GPR30 Shows Changes in mRNA Expression in the Rat Brain over the Oestrous Cycle
| Autor: | John K. Sinfield, J. C. Kaye, Trevor F.C. Batten, Sally E. Chapman, Emma J. Spary, Azhar Maqbool |
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| Rok vydání: | 2013 |
| Předmět: |
Male
GPR30 medicine.medical_specialty Oestrogen receptor Ovariectomy Hypothalamus Estrous Cycle Periaqueductal gray Biology lcsh:RC346-429 Receptors G-Protein-Coupled Cellular and Molecular Neuroscience Developmental Neuroscience Internal medicine medicine Animals RNA Messenger Ventrolateral medulla Rats Wistar Receptor lcsh:Neurology. Diseases of the nervous system Catecholaminergic Oestrous cycle lcsh:QP351-495 Solitary tract Autonomic Pathways Brain Nucleus tractus solitarius Rats lcsh:Neurophysiology and neuropsychology Endocrinology Gene Expression Regulation Receptors Estrogen nervous system Neurology Cholinergic Female GPER |
| Zdroj: | Neurosignals, Vol 21, Iss 1-2, Pp 14-27 (2012) |
| ISSN: | 1424-8638 1424-862X |
| DOI: | 10.1159/000333296 |
| Popis: | Oestrogen influences autonomic function via actions at classical nuclear oestrogen receptors α and β in the brain, and recent evidence suggests the orphan G protein-coupled receptor GPR30 may also function as a cytoplasmic oestrogen receptor. We investigated the expression of GPR30 in female rat brains throughout the oestrous cycle and after ovariectomy to determine whether GPR30 expression in central autonomic nuclei is correlated with circulating oestrogen levels. In the nucleus of the solitary tract (NTS), ventrolateral medulla (VLM) and periaqueductal gray (PAG) GPR30 mRNA, quantified by real-time PCR, was increased in proestrus and oestrus. In ovariectomised (OVX) rats, expression in NTS and VLM appeared increased compared to metoestrus, but in the hypothalamic paraventricular nucleus and PAG lower mRNA levels were seen in OVX. GPR30-like immunoreactivity (GPR30-LI) colocalised with Golgi in neurones in many brain areas associated with autonomic pathways, and analysis of numbers of immunoreactive neurones showed differences consistent with the PCR data. GPR30-LI was found in a variety of transmitter phenotypes, including cholinergic, serotonergic, catecholaminergic and nitrergic neurones in different neuronal groups. These observations support the view that GPR30 could act as a rapid transducer responding to oestrogen levels and thus modulate the activity of central autonomic pathways. |
| Databáze: | OpenAIRE |
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