Development of herpes simplex virus in the isolated nuclei of HEp-2 cells. II. Intranuclear events in isolated HSV-infected nuclei
| Autor: | E. H. Ludwig, D. L. Fine |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Time Factors
Arginine viruses Immunology Fluorescent Antibody Technique HSL and HSV Biology Immunofluorescence medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Cell Line Viral Proteins Cytopathogenic Effect Viral Antigen Genetics medicine Animals Humans Simplexvirus Microscopy Phase-Contrast Antigens Viral Laryngeal Neoplasms Molecular Biology Cell Nucleus Staining and Labeling medicine.diagnostic_test Immune Sera Carcinoma General Medicine Virology Molecular biology In vitro Culture Media Herpes simplex virus Microscopy Fluorescence DNA Viral Acridines Rabbits Deoxyribonuclease I |
| Zdroj: | Canadian Journal of Microbiology. 18:339-345 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m72-052 |
| Popis: | Specific immunofluorescence demonstrated a unique intranuclear fluorescent mass in incubated isolated herpes simplex virus (HSV)-infected HEp-2 nuclei. The intranuclear mass was observed to undergo a change in form, or maturation, during in vitro incubation when nuclei were isolated from HEp-2 cells 7 h post infection (p.i.). Both the accumulation and maturation of the intranuclear antigenic mass did not occur in HSV-infected nuclei isolated from HEp-2 cells grown in arginine-deficient medium. An accumulation of DNase-resistant acridine orange stainable aggregates occurred during in vitro incubation of nuclei isolated from 7- or 9-h HSV-infected cells. Enhanced nucleopathic effects did not occur under the same conditions of in vitro incubation. These data suggest that nuclei isolated from HSV-infected HEp-2 cells several hours p.i. are capable of allowing intranuclear virus development to continue under in vitro conditions in the absence of cytoplasmic contact. The findings may help to elucidate some of the roles of the cytoplasm and nucleus in herpes-virus development. |
| Databáze: | OpenAIRE |
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