Enkephalin and aFGF are differentially regulated in rat spinal motoneurons after chemodenervation with botulinum toxin
| Autor: | J.-M. Burgunder, Th Lauterburg, C. Pabst, A. M. Humm |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Botulinum Toxins Enkephalin Stilbamidines medicine.medical_treatment Vasoactive intestinal peptide Gene Expression Substance P Cell Count Galanin Biology Chemodenervation chemistry.chemical_compound Developmental Neuroscience Internal medicine medicine Animals Neuropeptide Y RNA Messenger Rats Wistar Muscle Skeletal Neurotensin Fluorescent Dyes Motor Neurons Age Factors Enkephalins Spinal cord Neuropeptide Y receptor Corpus Striatum Muscle Denervation Rats medicine.anatomical_structure Endocrinology Neurology chemistry Spinal Cord Nerve Degeneration Fibroblast Growth Factor 1 Female Axotomy Vasoactive Intestinal Peptide |
| Zdroj: | Experimental neurology. 161(1) |
| ISSN: | 0014-4886 |
| Popis: | Botulinum toxin is used to induce transient graded paresis by chemodenervation in the treatment of focal hyperkinetic movement disorders. While the molecular events occurring in motoneurons after mechanical nerve lesioning leading to muscle paresis are well known, they have been investigated to a lesser extent after chemodenervation. We therefore examined the expression of enkephalin (ENK), acidic fibroblast growth factor (aFGF), neurotensin (NT), galanin (GAL), substance P (SP), vasoactive intestinal polypeptide (VIP), and neuropeptide Y (NPY) in rat spinal motoneurons after chemodenervation of the gastrocnemius. In order to precisely localize the motoneurons targeting the injection site, retrograde tracing was performed in additional rats by using Fluorogold injections. ENK expression was upregulated in the region corresponding to the Fluorogold positive motoneurons, but also on the contralateral side and in more distant parts of the spinal cord. The highest upregulation occurred 7 to 14 days after injections and decreased over a period of three months. At 8 days, aFGF was slightly downregulated in all regions studied, single motoneurons showed NT expression, while expression of GAL, SP, VIP, and NPY could be detected neither in controls nor in toxin-treated animals. These alterations in gene expression were strikingly different from those described after axotomy. Our present findings give additional demonstration of the considerable plasticity of the adult spinal cord after botulinum toxin treatment. |
| Databáze: | OpenAIRE |
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