Biosynthesis of polyunsaturated fatty acids in the oleaginous marine diatom Fistulifera sp. strain JPCC DA0580
| Autor: | Yoshiaki Maeda, Masaki Muto, Yue Liang, Mitsufumi Matsumoto, Yoshihiko Sunaga, Tomoko Yoshino, Tsuyoshi Tanaka |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Fatty Acid Desaturases
eicosapentaenoic acid Chloroplasts Pharmaceutical Science Down-Regulation Endoplasmic Reticulum Article Metabolic engineering chemistry.chemical_compound Biosynthesis Fatty Acids Omega-6 Drug Discovery Fatty Acids Omega-3 Microalgae polyunsatulated fatty acid lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) chemistry.chemical_classification Diatoms biology Fatty acid food and beverages marine oleaginous diatom Fistulifera sp. JPCC DA0580 desaturase biology.organism_classification Eicosapentaenoic acid Chloroplast Diatom lcsh:Biology (General) chemistry Biochemistry Biodiesel production Biofuels Fatty Acids Unsaturated lipids (amino acids peptides and proteins) Transcriptome Polyunsaturated fatty acid |
| Zdroj: | Marine Drugs Marine Drugs; Volume 11; Issue 12; Pages: 5008-5023 Marine Drugs, Vol 11, Iss 12, Pp 5008-5023 (2013) |
| ISSN: | 1660-3397 |
| Popis: | Studies of polyunsaturated fatty acid (PUFA) biosynthesis in microalgae are of great importance for many reasons, including the production of biofuel and variable omega 3-long chain PUFAs. The elucidation of the PUFA biosynthesis pathway is necessary for bioengineering to increase or decrease PUFA content in certain microalgae. In this study, we identified the PUFA synthesis pathway in the oleaginous marine diatom, Fistulifera sp. strain JPCC DA0580, a promising candidate for biodiesel production. The data revealed not only the presence of the desaturases and elongases involved in eicosapentaenoic acid (EPA) synthesis, but also the unexpected localization of ω3-desaturase expression in the chloroplast. This suggests that this microalga might perform the final step of EPA synthesis in the chloroplast and not in the endoplasmic reticulum (ER) like other diatoms. The detailed fatty acid profile suggests that the EPA was synthesized only through the ω6-pathway in this strain, which was also different from other diatoms. Finally, the transcriptome analysis demonstrated an overall down-regulation of desaturases and elongases over incubation time. These genetic features might explain the decrease of PUFA percentage over incubation time in this strain. The important insights into metabolite synthesis acquired here will be useful for future metabolic engineering to control PUFA content in this diatom. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|