Mesorhizobium terrae sp. nov., a novel species isolated from soil in Jangsu, Korea
Autor: | Moonsuk Hur, Hyun Joong Kim, You-Jung Jung |
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Rok vydání: | 2020 |
Předmět: |
DNA
Bacterial Ubiquinone Biology Microbiology Plasmid RNA Ribosomal 16S Republic of Korea Mesorhizobium ciceri Molecular Biology Phospholipids Phylogeny Soil Microbiology Base Composition Strain (chemistry) Fatty Acids Mesorhizobium High-Throughput Nucleotide Sequencing General Medicine biology.organism_classification 16S ribosomal RNA Mesorhizobium loti Bacterial Typing Techniques Molecular Typing genomic DNA Genes Bacterial Sequence Analysis Bacteria |
Zdroj: | Antonie van Leeuwenhoek. 113(9) |
ISSN: | 1572-9699 |
Popis: | A gram-negative, white-pigmented, aerobic, rod-shaped bacterium, designated as strain NIBRBAC000500504T, was isolated from soil in Jangsu, Korea. Optimal growth of this strain was observed at 25 °C, pH 7.0, and in the presence of 0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NIBRBAC000500504T belonged to the genus Mesorhizobium and was closely related to Mesorhizobium shangrilense LMG 24762T (98.3% sequence similarity), Mesorhizobium australicum LMG 24608T (98.2%), Mesorhizobium qingshengii LMG 26793T (98.1%), Mesorhizobium ciceri ATCC 51585T (98.0%), Mesorhizobium loti DSM 2626T (98.0%), Mesorhizobium sophorae LMG 28223T (97.9%), Mesorhizobium waitakense LMG 28227T (97.8%), and Mesorhizobium cantuariense LMG 28225T (97.8%). Next-generation sequencing analysis indicated that the genome of strain NIBRBAC000500504T comprised a circular chromosome (5,731,152 bp, G+C content: 63.26%) and a plasmid (293,638 bp, G+C content: 61.39%) with 5672 coding sequences, 50 tRNAs, and 6 rRNAs. The major respiratory isoprenoid quinone was Q10; the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine; the major fatty acids were summed feature 8 (comprising C18:1 ω7c/C18:1 ω6c), C19:0 cyclo ω8c, C16:0, and C18:1 ω7c 11-methyl; and the G+C content of the genomic DNA was 62.9 mol%. The DNA-DNA relatedness values between NIBRBAC000500504T and its closest type strains were low. On the basis of these polyphasic taxonomic data, it is proposed that strain NIBRBAC000500504T represents a novel species of the genus Mesorhizobium, with the type strain being NIBRBAC000500504T (= KCTC 72278T = JCM 33432T). |
Databáze: | OpenAIRE |
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