Transcriptional activation is not responsible for increased levels of autonomously expressed simian virus 40 T-antigen in herpes simplex virus-infected cells
| Autor: | Edward D. Blair, B. Wendy Snowden, Edward K. Wagner |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
DNA Replication
Genetic Markers Transcription Genetic viruses Fluorescent Antibody Technique Simian virus 40 Biology Transfection medicine.disease_cause Antiviral Agents Marker gene Virus Cell Line Mice Plasmid Antigen Virology Genetics medicine Animals Simplexvirus Antigens Viral Tumor Promoter Regions Genetic Molecular Biology Gene Gene Amplification DNA replication General Medicine Precipitin Tests Molecular biology Herpes simplex virus Gene Expression Regulation Metals Superinfection DNA Viral Metallothionein Arabinonucleosides Plasmids Thymidine |
| Zdroj: | Virus Genes. 2:253-267 |
| ISSN: | 1572-994X 0920-8569 |
| DOI: | 10.1007/bf00125342 |
| Popis: | Herpes simples virus type 1 (HSV-1) superinfection of CV-1 cells weakly trans-activated a plasmid-borne metallothionein 1 (MT-1) promoter, but activated the expression of a marker gene controlled by an authentic HSV-1 promoter to a high level. In contrast, CMT-3 cells, which are CV-1 cells stably transformed with the simian virus 40 (SV40) large T-antigen (T-Ag) gene controlled by the MT-1 promoter, contained high levels of T-Ag following HSV-1 superinfection, but only if cells were preincubated in the presence of heavy-metal ions. This T-Ag was functional in that it could mediate the increase in copy number of a marker plasmid containing the SV40 origin of DNA replication. Pulse and continuous labeling of preinduced CMT-3 cells showed that T-Ag expression was not induced by HSV-1; but rather, HSV-1 superinfection resulted in the stabilization of pre-existing protein. |
| Databáze: | OpenAIRE |
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