Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya 'Huanong No. 1' in South China
| Autor: | Huaping Li, Cuiping Mo, Zilin Wu, Hengping Xie, Shuguang Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Veterinary medicine Population Population genetics genetically modified papaya Genetically modified crops Biology 010603 evolutionary biology 01 natural sciences Virus papaya leaf distortion mosaic virus 03 medical and health sciences evolutionary mechanism education Ecology Evolution Behavior and Systematics QH540-549.5 030304 developmental biology Nature and Landscape Conservation Original Research 0303 health sciences Genetic diversity education.field_of_study Phylogenetic tree Ecology Inoculation food and beverages Genetically modified organism genetic differentiation |
| Zdroj: | Ecology and Evolution, Vol 10, Iss 20, Pp 11671-11683 (2020) Ecology and Evolution |
| ISSN: | 2045-7758 |
| Popis: | The commercialized genetically modified papaya “Huanong No. 1” has been utilized to successfully control the destructive virus‐papaya ringspot virus (PRSV) in South China since 2006. However, another new emerging virus, papaya leaf distortion mosaic virus (PLDMV), was found in some PRSV‐resistant transgenic plants in Guangdong and Hainan provinces of South China through a field investigation from 2012 to 2019. The survey results showed that “Huanong No. 1” papaya plants are susceptible to PLDMV, and the disease prevalence in Hainan Province is generally higher than that in Guangdong Province. Twenty representative isolates were selected to inoculate “Huanong No. 1,” and all of the inoculated plants showed obvious disease symptoms similar to those in the field, indicating that PLDMV is a new threat to widely cultivated transgenic papaya in South China. Phylogenetic analysis of 111 PLDMV isolates in Guangdong and Hainan based on the coat protein nucleotide sequences showed that PLDMV isolates can be divided into two groups. The Japan and Taiwan China isolates belong to group I, whereas the Guangdong and Hainan isolates belong to group II and can be further divided into two subgroups. The Guangdong and Hainan isolates are far different from the Japan and Taiwan China isolates and belong to a new lineage. Further analysis showed that the Guangdong and Hainan isolates had a high degree of genetic differentiation, and no recombination was found. These isolates deviated from neutral evolution and experienced population expansion events in the past, which might still be unstable. The results of this study provide a theoretical basis for clarifying the evolutionary mechanism and population genetics of the virus and for preventing and controlling the viral disease. Papaya leaf distortion mosaic virus (PLDMV) has been reported in Japan and Taiwan, but the molecular evolution and population genetic structure of PLDMV have not been reported. This manuscript presents our recent findings that the isolates collected in this study have greatly diverged, indicating that they belong to a new lineage. Further analysis showed a highly genetic differentiation may contribute to difference of the host range. |
| Databáze: | OpenAIRE |
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