An eight-year study of Shigella species in Beijing, China: serodiversity, virulence genes, and antimicrobial resistance
| Autor: | Jie Li, Weili Liang, Mei Qu, Hanqiu Yan, Lei Jia, Quanyi Wang, Gui-rong Liu, Biao Kan, Xin Zhang, Ying Huang, Xiaona Wu, Xitai Li |
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| Rok vydání: | 2013 |
| Předmět: |
Serotype
China medicine.drug_class Antibiotics Virulence Shigella sonnei Microbial Sensitivity Tests Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Microbiology Antibiotic resistance Bacterial Proteins Virology Drug Resistance Bacterial medicine Shigella Agar diffusion test Longitudinal Studies Serotyping Gene Antigens Bacterial General Medicine Diarrhea Infectious Diseases Genes Bacterial Parasitology medicine.symptom |
| Zdroj: | Journal of infection in developing countries. 8(7) |
| ISSN: | 1972-2680 |
| Popis: | Introduction: This study was conducted to determine the prevalence of serotypes, virulence factors, and antimicrobial resistance patterns of Shigella spp. in Beijing, China, from 2004 to 2011. Methodology: Real-time PCR assays were used to detect virulent genes, and the Kirby-Bauer disk diffusion method was used to evaluate antimicrobial resistance. Results: Among the total of 1,652 Shigella isolates, S. sonnei (57.1%) was the predominant species, followed by S. flexneri (42.3%), S. dysenteriae (0.4%), and S. boydii (0.2%). Nineteen serotypes were discovered among S. flexneri strains. The virulence gene ipa H was the most frequent, followed by sen and set . The presence of set showed significant difference in two dominant serogroups, S. flexneri and S. sonnei . Over 90% of Shigella isolates showed resistance to at least three drugs with widened spectrum. High-level antimicrobial resistance to single and multiple antibiotics was more common among S. sonnei than S. flexneri . Conclusion: There was an obvious serotype change and a dramatic increase of antibiotic resistance in Shigella prevalence in Beijing. |
| Databáze: | OpenAIRE |
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