Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii
| Autor: | Yapei Zhao, Zhiqiang Hao, Tian Hu, Ruiqi Liu, Guoyan Liang, Guanglin Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine 01 natural sciences Catalysis Inorganic Chemistry Terpene lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Selaginella moellendorffii Pseudomonas syringae Physical and Theoretical Chemistry Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy stress physiology Methyl jasmonate ATP synthase biology Organic Chemistry General Medicine biology.organism_classification Terpenoid Computer Science Applications SmMTPSLs antibacterial 030104 developmental biology chemistry Biochemistry lcsh:Biology (General) lcsh:QD1-999 biology.protein GC-MS Function (biology) lycophyte 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 605, p 605 (2021) International Journal of Molecular Sciences Volume 22 Issue 2 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Selaginella moellendorffii is a lycophyte, a member of an ancient vascular plant lineage. Two distinct types of terpene synthase (TPS) genes were identified from this species, including S. moellendorffii TPS genes (SmTPSs) and S. moellendorffii microbial TPS-like genes (SmMTPSLs). The goal of this study was to investigate the biochemical functions of SmMTPSLs. Here, eight full-length SmMTPSL genes (SmMTPSL5, -15, -19, -23, -33, -37, -46, and -47) were functionally characterized from S. moellendorffii. Escherichia coli-expressed recombinant SmMTPSLs were tested for monoterpenes synthase and sesquiterpenes synthase activities. These enzymatic products were typical monoterpenes and sesquiterpenes that have been previous shown to be generated by typical plant TPSs when provided with geranyl diphosphate (GPP) and farnesyl diphosphate (FPP) as the substrates. Meanwhile, SmMTPSL23, -33, and -37 were up-regulated when induced by alamethicin (ALA) and methyl jasmonate (MeJA), suggesting a role for these genes in plants response to abiotic stresses. Furthermore, this study pointed out that the terpenoids products of SmMTPSL23, -33, and -37 have an antibacterial effect on Pseudomonas syringae pv. tomato DC3000 and Staphylococcus aureus. Taken together, these results provide more information about the catalytic and biochemical function of SmMTPSLs in S. moellendorffii plants. |
| Databáze: | OpenAIRE |
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