Streptomyces marianii sp. nov., a novel marine actinomycete from southern coast of India
| Autor: | Rajaretinam Rajesh Kannan, Chintalapati Venkata Ramana, Cathrin Spröer, Sam Albert Joshua, Chintalapati Sasikala, Wiebke Landwehr, Eedara Veera Venkata Ramprasad, Peter Schumann, Eby-Nesar Stella-Glory Deepak Shyl, Boyke Bunk, Francis-Joseph Rosemary Sharmila Joseph, Joachim Wink, Samuel Gnana Prakash Vincent, Appadurai Muthamil Iniyan |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology India Diamino acid 01 natural sciences Streptomyces Streptomyces wuyuanensis Microbiology 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Drug Discovery Streptomyces spongiicola Gene Indian Ocean Phylogeny Pharmacology biology Strain (chemistry) 010405 organic chemistry Dendrogram biology.organism_classification 16S ribosomal RNA 0104 chemical sciences RNA Bacterial chemistry |
| Zdroj: | The Journal of antibiotics. 74(1) |
| ISSN: | 1881-1469 |
| Popis: | A novel marine actinomycete strain designated ICN19T was isolated from the subtidal sediment of Chinnamuttam coast of Kanyakumari, India and subjected to polyphasic taxonomic analysis. Neighbour-joining tree based on 16S rRNA gene sequences of validly described type strains had revealed the strain ICN19T formed distinct cluster with Streptomyces wuyuanensis CGMCC 4.7042T, Streptomyces tirandamycinicus HNM0039T and Streptomyces spongiicola HNM0071T. Morphological, physiological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The strain possessed ll-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was identified as MK-9(H8) (70%), MK-9(H6) (20%) and MK-9(H2) (2%), with the major cellular fatty acids (>10%) being anteiso-C15:0, C16:0 and iso-C16:0. The main polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides and three unidentified phospholipids. The dendrogram generated on the basis of MALDI-TOF mass spectra supports the strain differentiated from its neigbours. The genome sequence of strain ICN19T was 9,010,366 bp in size with a total of 7420 protein-coding genes and 98 RNA genes. The genomic G+C content of the novel strain was 71.27 mol%. The DNA–DNA relatedness between strain ICN19T and the reference strains with S. wuyuanensis CGMCC 4.7042T, S. tirandamycinicus HNM0039T and S. spongiicola HNM0071T were 42.8%, 39.5% and 38%, respectively. Based on differences in physiological, biochemical, chemotaxonomic differences and whole-genome characteristics the isolated strain represents a novel species of the genus Streptomyces, for which the name Streptomyces marianii sp. nov. is proposed. Type strain is ICN19T (=MCC 3599T = KCTC 39749T). |
| Databáze: | OpenAIRE |
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