Type 3 Adenylyl Cyclase and Somatostatin Receptor 3 Expression Persists in Aged Rat Neocortical and Hippocampal Neuronal Cilia
| Autor: | Ashton Sequeira, Ronald J. Mandel, Ashok V. Kumar, Matthew R. Sarkisian, Gileno F. Filho, Thomas C. Foster, Susan L. Semple-Rowland, Sarah M. Guadiana, Alexander K. Parker, Gerry Shaw |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty cilium Cognitive Neuroscience Hippocampus Biology Hippocampal formation lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine Internal medicine Somatostatin receptor 3 medicine G protein-coupled receptor lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research Neocortex Dentate gyrus aging respiratory system 030104 developmental biology Endocrinology medicine.anatomical_structure nervous system Cerebral cortex Forebrain biology.protein cerebral cortex NeuN 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Aging Neuroscience Frontiers in Aging Neuroscience, Vol 8 (2016) |
| ISSN: | 1663-4365 |
| DOI: | 10.3389/fnagi.2016.00127 |
| Popis: | The primary cilia of forebrain neurons assemble around birth and become enriched with neuromodulatory receptors. Our understanding of the permanence of these structures and their associated signaling pathways in the aging brain is poor, but they are worthy of investigation because disruptions in neuronal cilia signaling have been implicated in changes in learning and memory, depression-like symptoms, and sleep anomalies. Here, we asked whether neurons in aged forebrain retain primary cilia and whether the staining characteristics of aged cilia for type 3 adenylyl cyclase (ACIII), somatostatin receptor 3 (SSTR3), and pericentrin resemble those of cilia in younger forebrain. To test this, we analyzed immunostained sections of forebrain tissues taken from young and aged male Fischer 344 (F344) and F344 × Brown Norway (F344 × BN) rats. Analyses of ACIII and SSTR3 in young and aged cortices of both strains of rats revealed that the staining patterns in the neocortex and hippocampus were comparable. Virtually every NeuN positive cell examined possessed an ACIII positive cilium. The lengths of ACIII positive cilia in neocortex were similar between young and aged for both strains, whereas in F344 × BN hippocampus, the cilia lengths increased with age in CA1 and CA3, but not in dentate gyrus (DG). Additionally, the percentages of ACIII positive cilia that were also SSTR3 positive did not differ between young and aged tissues in either strain. We also found that pericentrin, a protein that localizes to the basal bodies of neuronal cilia and functions in primary cilia assembly, persisted in aged cortical neurons of both rat strains. Collectively, our data show that neurons in aged rat forebrain possess primary cilia and that these cilia, like those present in younger brain, continue to localize ACIII, SSTR3, and pericentrin. Further studies will be required to determine if the function and signaling pathways regulated by cilia are similar in aged compared to young brain. |
| Databáze: | OpenAIRE |
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