Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate
| Autor: | Sung Gyun Kang, Jung-Hyun Lee, Hae-Chang Jung, Seong Hyuk Lee, Hyun Sook Lee, Sung-Mok Lee, Young Jun An |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular 0301 basic medicine Formates Science 030106 microbiology education Biology medicine.disease_cause Article Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Bioreactor medicine Formate Mutation Multidisciplinary Strain (chemistry) Chemiosmosis Point mutation Biological Transport Lyase biology.organism_classification Thermococcus Phenotype chemistry Biochemistry Medicine Carrier Proteins Genome Bacterial Genome-Wide Association Study Hydrogen |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) SCIENTIFIC REPORTS(7) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found among the genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formate transporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain, conferred increase in formate consumption and H2 production. Another adaptive passage, carried out by culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth. |
| Databáze: | OpenAIRE |
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