a-Smooth muscle actin and TGF-ß receptor I expression in the healing rabbit medial collateral and anterior cruciate ligaments

Autor: Giulio Gabbiani, Thomas Laumonier, Marie-Luce Bochaton-Piallat, Guido Garavaglia, Daniel Fritschy, Pierre Hoffmeyer, Jacques Menetrey
Rok vydání: 2011
Předmět:
Pathology
medicine.medical_specialty
Anterior cruciate ligament
Medial Collateral Ligament
Knee
Myofibroblasts/metabolism
ddc:616.07
03 medical and health sciences
0302 clinical medicine
Wound Healing/physiology
Medial Collateral Ligament
Knee/injuries/metabolism
Medicine
Animals
Anterior Cruciate Ligament
Receptor
Myofibroblasts
Actin
030304 developmental biology
General Environmental Science
030222 orthopedics
0303 health sciences
Medial collateral ligament
Wound Healing
ddc:617
business.industry
Anterior Cruciate Ligament Injuries
Muscle
Smooth
Anatomy
Anterior Cruciate Ligament/injuries/metabolism
musculoskeletal system
Actins
ddc:616.8
Tgf β receptors
medicine.anatomical_structure
α smooth muscle actin
Actins/metabolism
General Earth and Planetary Sciences
Female
Rabbits
business
Wound healing
Myofibroblast
Receptors
Transforming Growth Factor beta
human activities
Muscle
Smooth/injuries/metabolism
Receptors
Transforming Growth Factor beta/genetics/metabolism
Zdroj: Injury
Injury, Vol. 42, No 8 (2011) pp. 735-41
ISSN: 0020-1383
DOI: 10.1016/j.injury.2010.07.246
Popis: The aim of our study was to advance the knowledge about the biological differences in the healing of the anterior cruciate ligament (ACL) versus the medial collateral ligament (MCL). We quantified α-smooth muscle actin (α-SMA) expression and TGF-β receptor I (TGF-βRI) expression in experimentally injured rabbit ligaments (from day 3 to 12 weeks post-injury). Myofibroblasts (α-SMA positive cells) were identified as early as the third day post-injury in MCL and their density increased steadily up to day 21. Myofibroblasts were also detected in injured ACL but their density remained very low at all time points. The percentage of positive TGF-βRI area significantly increased in both injured ligaments compared to controls, with a peak expression at day 21; however, it remained constantly lower in ACL compared to MCL. A significant correlation was found between the percentage of TGF-βRI positive cells and the percentage of α-SMA expression only in injured MCL. These results provide evidence that myofibroblasts are important players in MCL remodelling after injury. The combined presence of myofibroblasts and TGF-βRI in the first 3 weeks post-MCL injury may partially explain the difference in the MCL and ACL healing process.
Databáze: OpenAIRE
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