Cysticercus bovis in cattle slaughtered in North Egypt: Overestimation by the visual inspection method
| Autor: | Nahla Salama, Naglaa Fathi Abd El-Latif, Mona H El-Sayad, Reda Fadly, Hoda Fahmy Farag, Hend A El-Taweel, Asmaa Abd Elhameed Ahmed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
040301 veterinary sciences 030231 tropical medicine cysticercus bovis polymerase chain reaction analysis Biology SF1-1100 World health law.invention 0403 veterinary science 03 medical and health sciences 0302 clinical medicine law parasitic diseases SF600-1100 medicine Helminths zoonotic Polymerase chain reaction General Veterinary taenia saginata food and beverages Cysticercosis 04 agricultural and veterinary sciences biology.organism_classification medicine.disease humanities Molecular analysis Animal culture Visual inspection cattle Taenia Cysticercus bovis Research Article |
| Zdroj: | Veterinary World, Vol 14, Iss 1, Pp 155-160 (2021) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Background and Aim: The World Health Organization and the Food and Agriculture Organization list Taenia saginata, a foodborne cestode, as the most widely distributed human tapeworm worldwide. The larval stage of T. saginata, Cysticercus bovis, causes cysticercosis in bovines and infects humans who eat raw or undercooked beef. The existing detection methods of C. bovis in cattle depend on the visual inspection of meat. This study aimed to confirm the identification of C. bovis through visual inspection at the slaughterhouses in North Egypt with a molecular diagnosis. Materials and Methods: A total of 687 locally bred cattle (Baladi), including 428 cows and 259 buffaloes, slaughtered in four slaughterhouses in North Egypt from April 2018 to February 2019 were inspected for C. bovis using the traditional meat inspection method. Positive samples were verified through polymerase chain reaction (PCR) amplification and HDP2 gene sequencing. Results: Through visual inspection, C. bovis was detected in 4.2% and 12.4% of the slaughtered cows and buffaloes, respectively. Molecular analysis confirmed that 1.9% of the animals, all of which were cows, had C. bovis infection. DNA sequencing verified the identity of the PCR-amplified product. Conclusion: The rate of C. bovis infection in slaughterhouses detected through meat inspection is overestimated compared with that through PCR. Although meat inspection can be used as a primary screening tool for C. bovis, a more specific molecular method is required to achieve an accurate diagnosis. |
| Databáze: | OpenAIRE |
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