A molecular epidemiological study of respiratory viruses detected in Japanese children with acute wheezing illness
| Autor: | Shumpei Yokota, Akihide Ryo, Atsuyoshi Nishina, Kazuko Goto-Sugai, Nobuhiko Okabe, Yoshimichi Okayama, Hiroyuki Tsukagoshi, Asako Fujitsuka, Mika Arakawa, Masato Tashiro, Masahiro Noda, Masakazu Yoshizumi, Masaaki Mori, Hirokazu Kimura, Katsumi Mizuta, Yoichi Kaburagi |
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| Jazyk: | angličtina |
| Předmět: |
Male
medicine.medical_specialty viruses Molecular Sequence Data medicine.disease_cause Virus lcsh:Infectious and parasitic diseases Medical microbiology Japan Human metapneumovirus medicine Humans lcsh:RC109-216 Respiratory Tract Infections Phylogeny Respiratory Sounds Asthma Molecular Epidemiology biology Respiratory tract infections Molecular epidemiology Infant virus diseases respiratory system biology.organism_classification medicine.disease Virology Human Parainfluenza Virus Infectious Diseases Child Preschool Viruses Immunology Female Rhinovirus Research Article |
| Zdroj: | BMC Infectious Diseases, Vol 11, Iss 1, p 168 (2011) BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| DOI: | 10.1186/1471-2334-11-168 |
| Popis: | Background Recent studies strongly suggest that some respiratory viruses are associated with the induction of acute wheezing and/or exacerbation of bronchial asthma. However, molecular epidemiology of these viruses is not exactly known. Methods Using PCR technology, we attempted to detect various respiratory viruses from 115 Japanese children. Furthermore, the detected viruses were subjected to homology, pairwise distance, and phylogenetic analysis. Results Viruses were detected from 99 (86.1%) patients. Respiratory syncytial virus (RSV) alone and human rhinovirus (HRV) alone were detected in 47 (40.9%) and 36 (31.3%) patients, respectively. Both RSV and HRV were detected in 14 (12.2%) patients. Human metapneumovirus (HMPV) alone and human parainfluenza virus (HPIV) alone were detected in 1 (0.9%) patient each, respectively. Homology and phylogenetic analyses showed that the RSV and HRV strains were classified into genetically diverse species or subgroups. In addition, RSV was the dominant virus detected in patients with no history of wheezing, whereas HRV was dominant in patients with a history of wheezing. Conclusions The results suggested that these genetically diverse respiratory viruses, especially RSV and HRV, might be associated with wheezing in Japanese children. |
| Databáze: | OpenAIRE |
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