Vα domain modulates the multiple topologies of mouse T cell receptor Vβ20/staphylococcal enterotoxins A and E complexes
| Autor: | Y Bravo de Alba, Pierre-André Cazenave, Rafick Pierre Sekaly, Isabelle Cloutier, J Thibodeau, Patrice N. Marche |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Receptors
Antigen T-Cell alpha-beta T-Lymphocytes T cell Immunology Antigen-Presenting Cells chemical and pharmacologic phenomena Biology Lymphocyte Activation Transfection Major histocompatibility complex Enterotoxins Mice Structure-Activity Relationship Superantigen medicine Animals Humans Immunology and Allergy Antigen-presenting cell Genetics MHC class II T-cell receptor HLA-DR1 Antigen Molecular biology Recombinant Proteins medicine.anatomical_structure biology.protein Alpha chain CD8 Protein Binding |
| Zdroj: | European Journal of Immunology. 27:92-99 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830270114 |
| Popis: | The superantigens staphylococcal enterotoxin A and E (SEA and SEE) both contact major histocompatibility complex (MHC) class II molecules on two sites located on the alpha and beta chains. We have investigated the role of the T cell receptor (TCR) alpha chain in the modulation of the various topologies of TCR/SEA (or SEE)/class II complexes. For this purpose, we have used three mouse V beta20 T cell lines expressing different V alpha domains and two T cell hybridomas expressing mouse V beta1 or V beta11 segments. The response of these T cells to SEA and SEE was studied in the context of presentation by wild-type human MHC class II molecules; or by mutants on MHC, in each of the two superantigen binding sites (position alpha39K and beta81H) to which the superantigens can still bind but with an altered conformation. Although V beta20 T cell lines are efficiently stimulated using SEA and SEE presented by wild-type HLA-DR1 molecules, our results show that the nature of the TCR V alpha domain can affect differently the recognition of the toxins bound to mutant class II molecules. This suggests that various functional topologies exist for both SEA and SEE/class II complexes and that the T cell response to each of these complexes can be modulated by the V alpha domain of the TCR. Interestingly, the recognition of SEA and SEE is achieved in different fashions by a given V beta20 T cell line. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text