MUB40 Binds to Lactoferrin and Stands as a Specific Neutrophil Marker

Autor: Friederike Jönsson, Mariette Matondo, Dorothée Selimoglu-Buet, Benoit S. Marteyn, Bruno Baron, Naelle Lombion, Philippe J. Sansonetti, Jean-Yves Tinevez, Giulia Nigro, Thierry Lazure, Yves Marie Coïc, Valérie Lapierre, David J. Thornton, Katharina Nothelfer, Mark C. Anderson, Louise Injarabian, Thibault Chaze, Françoise Baleux, Ellen T. Arena, Eric Solary, Judith Souphron, Pascale Vonaesch, Caroline Ridley
Přispěvatelé: Pathogénie microbienne moléculaire, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Plateforme de Protéomique / Proteomics platform, Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Chimie des Biomolécules - Chemistry of Biomolecules, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Laboratoire de thérapie cellulaire, Département de médecine oncologique [Gustave Roussy], Institut Gustave Roussy (IGR)-Institut Gustave Roussy (IGR), Hématopoïèse normale et pathologique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Gustave Roussy (IGR)-Université Paris-Sud - Paris 11 (UP11), University of Manchester [Manchester], Biophysique Moléculaire (Plate-forme), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Imagopole (CITECH), Institut Pasteur [Paris], AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Département d'Immunologie - Department of Immunology, Chaire Microbiologie et Maladies infectieuses, Collège de France (CdF (institution)), This work was supported by the Fondation Laurette Fugain (LF-2015-15) (B.S.M.) and ANR JCJC grants (ANR-17-CE15-0012) (B.S.M.) - (ANR-16-CE15-0012-01) (F.J.)., ANR-17-CE15-0012,NEUTROXIA,Effet de l'exposition des neutrophiles à l'oxygène sur leur activation et leur mort : une lame à double tranchant(2017), ANR-16-CE15-0012,PlanA,Rôle des plaquettes et leurs interactions dans les réactions anaphylactiques(2016), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Collège de France - Chaire Microbiologie et Maladies infectieuses
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
MESH: Inflammation
Clinical Biochemistry
Cell
Arthritis
MESH: Rabbits
Peptide
MESH: Neutrophils
Biochemistry
MESH: Shigella
MUB
Drug Discovery
MESH: Animals
chemistry.chemical_classification
MESH: Middle Aged
Lactoferrin
MESH: Peptides
imaging
neutrophil
MESH: Fluorescent Dyes
3. Good health
lactoferrin
medicine.anatomical_structure
Molecular Medicine
biomarker
[SDV.IMM]Life Sciences [q-bio]/Immunology
MESH: Carbocyanines
medicine.symptom
MESH: Dysentery
Bacillary
Inflammation
Biology
MESH: Guinea Pigs
Guinea pig
03 medical and health sciences
Immune system
MESH: Mice
Inbred C57BL
medicine
Secretion
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Molecular Biology
MESH: Mice
Pharmacology
MESH: Humans
MUB(40)
MESH: Adult
medicine.disease
Molecular biology
MESH: Lactoferrin
030104 developmental biology
chemistry
biology.protein
MESH: Biomarkers
MESH: Female
Zdroj: Cell Chemical Biology
Cell Chemical Biology, Cell Press, 2018, 25 (4), pp.483-493.e9. ⟨10.1016/j.chembiol.2018.01.014⟩
Cell Chemical Biology, 2018, 25 (4), pp.483-493.e9. ⟨10.1016/j.chembiol.2018.01.014⟩
Anderson, M C, Chaze, T, Coïc, Y M, Injarabian, L, Jonsson, F, Lombion, N, Selimoglu-Buet, D, Souphron, J, Ridley, C, Vonaesch, P, Baron, B, Arena, E T, Tinevez, J Y, Nigro, G, Nothelfer, K, Solary, E, Lapierre, V, Lazure, T, Matondo, M, Thornton, D, Sansonetti, P J, Baleux, F & Marteyn, B S 2018, ' MUB 40 Binds to Lactoferrin and Stands as a Specific Neutrophil Marker ', Cell Chemical Biology, vol. 25, no. 4, pp. 483-493.e9 . https://doi.org/10.1016/j.chembiol.2018.01.014
ISSN: 2451-9456
DOI: 10.1016/j.chembiol.2018.01.014⟩
Popis: Neutrophils represent the most abundant immune cells recruited to inflamed tissues. A lack of dedicated tools has hampered their detection and study. We show that a synthesized peptide, MUB40, binds to lactoferrin, the most abundant protein stored in neutrophil-specific and tertiary granules. Lactoferrin is specifically produced by neutrophils among other leukocytes, making MUB40 a specific neutrophil marker. Naive mammalian neutrophils (human, guinea pig, mouse, rabbit) were labeled by fluorescent MUB40 conjugates (-Cy5, Dylight405). A peptidase-resistant retro-inverso MUB40 (RI-MUB40) was synthesized and its lactoferrin-binding property validated. Neutrophil lactoferrin secretion during in vitro Shigella infection was assessed with RI-MUB40-Cy5 using live cell microscopy. Systemically administered RI-MUB40-Cy5 accumulated at sites of inflammation in a mouse arthritis inflammation model in vivo and showed usefulness as a potential tool for inflammation detection using non-invasive imaging. Improving neutrophil detection with the universal and specific MUB40 marker will aid the study of broad ranges of inflammatory diseases. MUB40 is a universal and specific marker of mammalian neutrophils. MUB40 interacts with lactoferrin, stored in specific (β1), tertiary (β2), and secretome (γ) granules secreted upon stimulation. Neutrophil detection in inflamed or infected tissues is facilitated by MUB40 peptides conjugated to fluorophores. Neutrophilic inflammation detection may be envisaged by combining MUB40 with non-invasive imaging.
Databáze: OpenAIRE
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