A natural mutation in Pisum sativum L. (pea) alters starch assembly and improves glucose homeostasis in humans

Autor: Mai Khatib, Yaroslav Z. Khimyak, Jose Ivan Serrano-Contreras, Tom Preston, Frederick J. Warren, Lee Kellingray, Todor Koev, Elaine Holmes, Cathrina H. Edwards, Arjan Narbad, Louise J. Salt, Rachael Stanley, Rasha Alshaalan, Maria N. Charalambides, Katerina Petropoulou, Claire Domoney, Julian Marchesi, Kathryn Cross, Jesus Miguens Blanco, Ian F. Godsland, Peter J. Wilde, Nicholas Penney, Natalia Perez-Moral, Gary Frost, Douglas J. Morrison, Edward S. Chambers, Rocio Castro Seoane, Isabel Garcia-Perez, Julie A. K. McDonald
Jazyk: angličtina
Rok vydání: 2020
Předmět:
ISSN: 2662-1355
Popis: Elevated postprandial glucose (PPG) is a significant risk factor for non-communicable diseases globally. Currently, there is a limited understanding of how starch structures within a carbohydrate-rich food matrix interact with the gut luminal environment to control PPG. Here, we use pea seeds (Pisum sativum) and pea flour, derived from two near-identical pea genotypes (BC1/19RR and BC1/19rr) differing primarily in the type of starch accumulated, to explore the contribution of starch structure, food matrix and intestinal environment to PPG. Using stable isotope 13C-labelled pea seeds, coupled with synchronous gastric, duodenal and plasma sampling in vivo, we demonstrate that maintenance of cell structure and changes in starch morphology are closely related to lower glucose availability in the small intestine, resulting in acutely lower PPG and promotion of changes in the gut bacterial composition associated with long-term metabolic health improvements. Seeds, flour and food products derived from two near-identical pea genotypes (BC1/19RR and BC1/19rr) were utilized in a series of in vitro, ex vivo and in vivo studies to explore the contribution of starch structure, food matrix and intestinal environment to postprandial glycaemia.
Databáze: OpenAIRE
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