c-Maf plays a crucial role for the definitive erythropoiesis that accompanies erythroblastic island formation in the fetal liver
| Autor: | Takayuki Ohsumi, Kazuhiko Uchida, Kazuteru Hasegawa, Satoru Takahashi, Haruhiko Ninomiya, Shigeru Chiba, Michito Hamada, Takashi Kudo, Hirona Suzuki, Tran Thi Nhu Mai, Megumi Nakamura, Manabu Kusakabe |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Erythroblasts Liver cytology Immunology Cell Communication Biology Biochemistry Andrology Mice Fetus Cell Movement Internal medicine medicine Animals Erythropoiesis Fibrosarcoma Transcription factor Cell Proliferation Mice Knockout Cell growth Microarray analysis techniques Gene Expression Profiling Macrophages Embryo Cell Biology Hematology Embryo Mammalian Microarray Analysis medicine.disease Embryonic stem cell Mice Inbred C57BL Endocrinology Liver Proto-Oncogene Proteins c-maf |
| Zdroj: | Blood. 118:1374-1385 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2010-08-300400 |
| Popis: | c-Maf is one of the large Maf (musculoaponeurotic fibrosarcoma) transcription factors that belong to the activated protein-1 super family of basic leucine zipper proteins. Despite its overexpression in hematologic malignancies, the physiologic roles c-Maf plays in normal hematopoiesis have been largely unexplored. On a C57BL/6J background, c-Maf−/− embryos succumbed from severe erythropenia between embryonic day (E) 15 and E18. Flow cytometric analysis of fetal liver cells showed that the mature erythroid compartments were significantly reduced in c-Maf−/− embryos compared with c-Maf+/+ littermates. Interestingly, the CFU assay indicated there was no significant difference between c-Maf+/+ and c-Maf−/− fetal liver cells in erythroid colony counts. This result indicated that impaired definitive erythropoiesis in c-Maf−/− embryos is because of a non–cell-autonomous effect, suggesting a defective erythropoietic microenvironment in the fetal liver. As expected, the number of erythroblasts surrounding the macrophages in erythroblastic islands was significantly reduced in c-Maf−/− embryos. Moreover, decreased expression of VCAM-1 was observed in c-Maf−/− fetal liver macrophages. In conclusion, these results strongly suggest that c-Maf is crucial for definitive erythropoiesis in fetal liver, playing an important role in macrophages that constitute erythroblastic islands. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|