Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa
| Autor: | Cantero, Jon, Grau-Expósito, Judith, Serra-Peinado, Carla, Rosero, D. A., Luque-Ballesteros, Laura, Astorga-Gamaza, Antonio, Castellvi, Josep., Sanhueza, T., Tapia Melendo, Gustavo, Lloveras, B., Fernández, Marco A., Prado, Julia G., Solé-Sedeno, J. M., Tarrats, A., Lecumberri, Carla, Mañalich-Barrachina, L., Centeno-Mediavilla, C., Falcó, Vicenç, Buzón, Maria José, Genescà Ferrer, Meritxell., Universitat Autònoma de Barcelona |
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| Přispěvatelé: | [Cantero-Pérez J, Grau-Expósito J, Serra-Peinado C, Rosero DA, Luque-Ballesteros L, Astorga-Gamaza A, Falcó V, Buzon MJ, Genescà M] Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Grup de Recerca en Malalties Infeccioses, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Castellví J] Servei d’Anatomia Patològica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Mañalich-Barrachina L, Centeno-Mediavilla C] Servei de Ginecologia i Obstetrícia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Rok vydání: | 2019 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine CD32 Human immunodeficiency virus (HIV) Urogenital System::Genitalia::Genitalia Female::Uterus::Cervix Uteri [ANATOMY] General Physics and Astronomy HIV Infections Cervix Uteri medicine.disease_cause Pathogenesis 0302 clinical medicine Coll uterí sistema urogenital::genitales::genitales femeninos::útero::cuello del útero [ANATOMÍA] Dna viral lcsh:Science Multidisciplinary biology virus diseases Middle Aged Viral Load Phenotype 3. Good health Cèl·lules T Anti-Retroviral Agents Female HIV infections Adult Female circumcision Science Article General Biochemistry Genetics and Molecular Biology Viral reservoirs 03 medical and health sciences enfermedades del sistema inmune::síndromes de inmunodeficiencia::infecciones por VIH [ENFERMEDADES] VIH (Virus) medicine Humans Mucosa cervical Aged Disease Reservoirs Hemic and Immune Systems::Hemic and Immune Systems::Immune System::Leukocytes::Leukocytes Mononuclear::Lymphocytes::T-Lymphocytes::CD4-Positive T-Lymphocytes [ANATOMY] Mucous Membrane Cluster of differentiation RNA General Chemistry Virology Immune System Diseases::Immunologic Deficiency Syndromes::HIV Infections [DISEASES] sistemas sanguíneo e inmunológico::sistemas sanguíneo e inmunológico::sistema inmunológico::leucocitos::leucocitos mononucleares::linfocitos::linfocitos T::linfocitos T CD4-positivos [ANATOMÍA] 030104 developmental biology HIV-1 biology.protein Infeccions per VIH lcsh:Q Immunologic Memory 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019) Scientia |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-12732-2 |
| Popis: | HIV viral reservoirs are established very early during infection. Resident memory T cells (TRM) are present in tissues such as the lower female genital tract, but the contribution of this subset of cells to the pathogenesis and persistence of HIV remains unclear. Here, we show that cervical CD4+TRM display a unique repertoire of clusters of differentiation, with enrichment of several molecules associated with HIV infection susceptibility, longevity and self-renewing capacities. These protein profiles are enriched in a fraction of CD4+TRM expressing CD32. Cervical explant models show that CD4+TRM preferentially support HIV infection and harbor more viral DNA and protein than non-TRM. Importantly, cervical tissue from ART-suppressed HIV+ women contain high levels of viral DNA and RNA, being the TRM fraction the principal contributor. These results recognize the lower female genital tract as an HIV sanctuary and identify CD4+TRM as primary targets of HIV infection and viral persistence. Thus, strategies towards an HIV cure will need to consider TRM phenotypes, which are widely distributed in tissues. Using cervical explant models and cervical tissue from ART-suppressed HIV+ women, the authors here show that resident memory T cells (TRM) in the cervical mucosa are preferentially infected and harbor more viral DNA, RNA and protein than non-TRM, suggesting that TRM may serve as viral reservoir in the cervical mucosa. |
| Databáze: | OpenAIRE |
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