Infectivity of patent Plasmodium falciparum gametocyte carriers to mosquitoes: establishing capacity to investigate the infectious reservoir of malaria in a low-transmission setting in The Gambia
| Autor: | Jane Achan, Harouna M Soumare, Pa Modou Gaye, Abdullahi Ahmad, John S. Bradley, Teun Bousema, Umberto D'Alessandro, Chris Drakeley, Haddy Bittaye, Lamin Jadama, Marta Moreno, Muhammed M. Camara |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Future studies Plasmodium falciparum gametocytes 030231 tropical medicine Low transmission 03 medical and health sciences 0302 clinical medicine Anopheles parasitic diseases medicine Gametocyte Animals Humans AcademicSubjects/MED00860 Malaria Falciparum Infectivity Plasmodium falciparum gametocyte biology direct membrane feeding assays Transmission (medicine) Public Health Environmental and Occupational Health Bayes Theorem General Medicine medicine.disease biology.organism_classification Virology Anopheles coluzzii The Gambia Malaria AcademicSubjects/MED00290 lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] 030104 developmental biology Infectious Diseases Gambia Original Article Parasitology Human medicine |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene, 115, 1462-1467 Transactions of the Royal Society of Tropical Medicine and Hygiene, 115, 12, pp. 1462-1467 Transactions of the Royal Society of Tropical Medicine and Hygiene |
| ISSN: | 0035-9203 |
| Popis: | Background Understanding the human malaria infectious reservoir is important for elimination initiatives. Here, we implemented mosquito membrane feeding experiments to prepare for larger studies to quantify the transmission potential and relative contribution of the human infectious reservoir. Methods Patients with clinical malaria attending four health facilities with at least 16 Plasmodium falciparum gametocytes per μL were recruited during the 2018 transmission season. Infectiousness to mosquitoes was assessed by direct membrane feeding assay (DMFA). We compared our results with a Bayesian predictive model to investigate the relationship between infectiousness and gametocyte density and explore the impact of fever on gametocyte infectivity. Results A total of 3177 suspected malaria cases were screened; 43.3% (1376) had microscopically patent P. falciparum parasites and 3.6% (114) of them had gametocytes. Out of 68 DMFAs, 38 (55.9%) resulted in at least one infected mosquito, with a total of 15.4% (1178/7667) of mosquitoes infected with 1–475 oocysts per gut. The relationship between mosquito infection prevalence and gametocytaemia was similar to other African settings and negatively associated with fever (OR: 0.188, 95% CI 0.0603 to 0.585, p=0.0039). Conclusions Among symptomatic malaria patients, fever is strongly associated with transmission failure. Future studies can use DMFA to better understand the human malaria reservoir in settings of low endemicity in The Gambia and inform malaria elimination initiatives. |
| Databáze: | OpenAIRE |
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