Electron Capture Dissociation in a Radio Frequency Ion Trap

Autor:	and Atsumu Hirabayashi, Izumi Waki, Yuichiro Hashimoto, Hideki Hasegawa, Takashi Baba
Rok vydání:	2004
Předmět:	Electron-capture dissociation Radio Waves Electron capture Chemistry Electrons Electron Dissociation (chemistry) Analytical Chemistry Kinetics Spectroscopy Fourier Transform Infrared Ion trap Irradiation Radio frequency Atomic physics Peptides Radio wave
Zdroj:	Analytical Chemistry. 76:4263-4266
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac049309h
Popis:	We report on the first evidence of electron capture dissociation (ECD) in a radio frequency (rf) ion trap. Peptide ions, [substance P]2+, trapped in a two-dimensional, linear rf ion trap were cleaved by electrons injected along the central axis of the trap. Along the axis, the rf field component was zero and a magnetic field of 50 mT was applied. This electron injection scheme keeps the energy of the electrons below 1 eV, preventing them from heating by the rf field. The present ECD efficiency is approximately 4% by irradiation of electron current of 0.2 microA for 80 ms. ECD in rf traps may open high-throughput and low-cost ECD applications to obtain molecular structure information complementary to collision-induced dissociation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8390580bef03a29988a9197e402f81b https://doi.org/10.1021/ac049309h Zobrazit plný text záznamu