Formulation of liposomes loading lentisk oil to ameliorate topical delivery, attenuate oxidative stress damage and improve cell migration in scratch assay

Autor: Elvira Escribano-Ferrer, Mohamad Allaw, Octavio Díez-Sales, Maria Manconi, Maria Letizia Manca, Pierluigi Caboni, Gianluigi Bacchetta, Amparo Nácher, José Esteban Peris
Rok vydání: 2021
Předmět:
Keratinocytes
Swine
Administration
Topical
medicine.disease_cause
law.invention
chemistry.chemical_compound
Mice
Skin absorption
law
Cell Movement
Scratch assay
Lecithins
Materials Testing
Food science
Hydrogen peroxide
Wound healing
Scratch assay
Flavor
Liposome
Skin permeation studies
biology
Vesicle
Pell
General Medicine
Oxidants
Pistacia
Keratinocytes & fibroblasts
Pistacia lentiscus
Farmacologia
Drug Compounding
Wound healing
RM1-950
Cell Line
medicine
Oils
Volatile
Animals
Humans
Absorció cutània
Particle Size
Essential oil
Pharmacology
Olis essencials
Penetration (firestop)
Hydrogen Peroxide
biology.organism_classification
Confocal microscopy
Oxidative Stress
chemistry
Essences and essential oils
Liposomes
Skin permeation studies
confocal microscopy
Therapeutics. Pharmacology
Soybeans
Oxidative stress
Zdroj: Allaw, Mohamad Manconi, Maria Caboni, Pierluigi Bacchetta, Gianluigi Escribano-Ferrer, Elvira Peris Ribera, José Esteban Nácher Alonso, Amparo Díez Sales, Octavio Manca, Maria Letizia 2021 Formulation of liposomes loading lentisk oil to ameliorate topical delivery, attenuate oxidative stress damage and improve cell migration in scratch assay Biomedicine & Pharmacotherapy 144 112351
Digital.CSIC. Repositorio Institucional del CSIC
instname
Biomedicine & Pharmacotherapy, Vol 144, Iss, Pp 112351-(2021)
RODERIC. Repositorio Institucional de la Universitat de Valéncia
Popis: [EN] Pistacia lentiscus L. is a sclerophyllous shrub capable of growing under harsh climatic conditions especially in the Mediterranean Basin. Different products can be obtained from this plant, such as essential oil, mastic gum or even fixed oil. The last is well known for its flavor which is mainly exploited in the food industry. Additionally, it has been traditionally used in the treatment of skin diseases, but, at the moment, any suitable formulation for skin delivery has been formulated and its biological effects was not deeply confirmed. Given that, in the present study, the lentisk oil has been formulated in liposomes at different concentrations (10, 20, 30 mg/ml) and their physicochemical, technological and main biological properties have been evaluated. Vesicles were prepared by using natural soy lecithin and a green and organic solvent free method, thus obtaining spherical, small (similar to 118 nm), homogeneously dispersed (0.27) and highly negatively charged (similar to -62 mV) vesicles. The used amount of oil loaded in liposomes (10, 20, 30 mg/ml) modulated the penetration ability of vesicles in the skin, favoring the deposition of the payload in the deeper strata. The loading in the vesicles potentiated the ability of oil to counteract the damaging effects caused by hydrogen peroxide in keratinocytes and fibroblasts and facilitate their migration in a cell monolayer lesion. Overall findings suggested that the incorporation of lentisk oil in liposomes made from soy lecithin can be an alternative and natural approach to exploit it in pharmaceutical ad cosmetical applications and manufacturing natural products suitable for the treatment of skin lesions.
The authors thank MEDLENTISK project "Partnership for the exchange of best practices on lentisk tree fixed oil, an emblematic non-timber forest product in the Mediterranean" co-funded by the Erasmus+ Programme of the European Union for providing analytical equipment and materials.
Databáze: OpenAIRE
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