Proteome profiling in the aorta and kidney of type 1 diabetic rats

Autor: Ayad A. Jaffa, Fuad N. Ziyadeh, Miran A. Jaffa, Parvin Mirzaei, Mohamad Elmedawar, Jingfu Zhao, Moustafa Al Hariri, Rui Zhu, Firas Kobeissy, Yehia Mechref, Adnan Ahmed
Rok vydání: 2017
Předmět:
0301 basic medicine
Blood Glucose
Proteomics
medicine.medical_treatment
Protein Expression
lcsh:Medicine
Kidney
Biochemistry
Endocrinology
Tandem Mass Spectrometry
Medicine and Health Sciences
Insulin
lcsh:Science
Aorta
Macrovascular disease
Multidisciplinary
Organic Compounds
Monosaccharides
Acute-phase protein
STAT proteins
Chemistry
medicine.anatomical_structure
Physical Sciences
Anatomy
Research Article
medicine.medical_specialty
Endocrine Disorders
Carbohydrates
Biology
Peptidyl-Dipeptidase A
Research and Analysis Methods
Gene Expression Regulation
Enzymologic
03 medical and health sciences
Downregulation and upregulation
Internal medicine
Diabetes mellitus
medicine
Diabetes Mellitus
Gene Expression and Vector Techniques
Animals
Molecular Biology Techniques
Molecular Biology
Diabetic Endocrinology
Molecular Biology Assays and Analysis Techniques
Kininogens
lcsh:R
Body Weight
Organic Chemistry
Chemical Compounds
Kidney metabolism
Biology and Life Sciences
Proteins
Kidneys
Renal System
medicine.disease
Hormones
Rats
Oxidative Stress
030104 developmental biology
Diabetes Mellitus
Type 1
Glucose
Metabolic Disorders
Cardiovascular Anatomy
Blood Vessels
lcsh:Q
Protein Processing
Post-Translational
Chromatography
Liquid
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 11, p e0187752 (2017)
ISSN: 1932-6203
Popis: Diabetes is associated with a number of metabolic and cardiovascular risk factors that contribute to a high rate of microvascular and macrovascular complications. The risk factors and mechanisms that contribute to the development of micro- and macrovascular disease in diabetes are not fully explained. In this study, we employed mass spectrometric analysis using tandem LC-MS/MS to generate a proteomic profile of protein abundance and post-translational modifications (PTM) in the aorta and kidney of diabetic rats. In addition, systems biology analyses were employed to identify key protein markers that can provide insights into molecular pathways and processes that are differentially regulated in the aorta and kidney of type 1 diabetic rats. Our results indicated that 188 (111 downregulated and 77 upregulated) proteins were significantly identified in the aorta of diabetic rats compared to normal controls. A total of 223 (109 downregulated and 114 upregulated) proteins were significantly identified in the kidney of diabetic rats compared to normal controls. When the protein profiles from the kidney and aorta of diabetic and control rats were analyzed by principal component analysis, a distinct separation of the groups was observed. In addition, diabetes resulted in a significant increase in PTM (oxidation, phosphorylation, and acetylation) of proteins in the kidney and aorta and this effect was partially reversed by insulin treatment. Ingenuity pathway analysis performed on the list of differentially expressed proteins depicted mitochondrial dysfunction, oxidative phosphorylation and acute phase response signaling to be among the altered canonical pathways by diabetes in both tissues. The findings of the present study provide a global proteomics view of markers that highlight the mechanisms and putative processes that modulate renal and vascular injury in diabetes.
Databáze: OpenAIRE
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