Reference values of IGF-I in children from birth to 5 years of age, in Burkina Faso, using blood samples on filter paper
| Autor: | B. Doulougou, René Tonglet, Jean-Marie Ketelslegers, Annie Robert, V. De Coninck, B. Sondo, Séni Kouanda |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Blood Specimen Collection Pediatrics medicine.medical_specialty Hematologic Tests business.industry Micropore Filters Endocrinology Diabetes and Metabolism Infant Newborn Infant Nutritional Status Dried blood spot Endocrinology Social Class African population Reference Values Child Preschool Reference values Burkina Faso Humans Medicine Female Insulin-Like Growth Factor I business |
| Zdroj: | Growth Hormone & IGF Research. 18:345-352 |
| ISSN: | 1096-6374 |
| Popis: | The aims of this study were to validate the use of filter paper to measure insulin-like growth factor-I (IGF-I) and to establish normal levels of IGF-I in children appearing healthy, from birth to 5 years of age in an African population.We determined IGF-I from blood collected on filter paper. We validated this method by comparing the IGF-I values from dried blood spots on filter paper (kept at 4 degrees C and ambient temperature) and from serum among 13 children under 5. IGF-I were measured by the classical IGF-I RIA, after separation of the IGF-I from its binding proteins, using Sep-Pak chromatography. To establish normal levels of IGF-I, we conducted a cross-sectional study and collected blood samples with filter paper among 360 children in Ouagadougou (Burkina Faso).IGF-I determined from dried blood spots on filter paper were in good agreement with IGF-I levels obtained from blood serum, whether the filter papers were kept at 4 degrees C or at ambient temperature. The results of IGF-I-levels in apparently healthy children showed that geometric mean IGF-I ranged from 27 microg/l in boys younger than five months to 31 microg/l in 5-year-old boys. In girls, mean IGF-I ranged from 29 microg/l for girls younger than five months to 45 microg/l at the age of 5. From birth to 24 months, IGF-I decreased by 0.32+/-0.08 microg/l/month in boys and by 0.27+/-0.06 microg/l/month in girls and these decreases were not significantly different (p=0.95). After the age of 24 months, there was an increase in IGF-I of 4.9+/-1.3 microg/l/year in boys and of 8.4+/-0.8 microg/l/year in girls. This increase was indeed significantly different (p0.001).Reference values of IGF-I for African boys and girls were determined. They will be used for endocrine evaluations and nutritional monitoring. |
| Databáze: | OpenAIRE |
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