The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
| Autor: | Zhiwen Pan, Xiaohong Xu, Yejiang Bao, Weimin Mao, Xinhua Su, Min Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Esophageal Neoplasms Gene Expression Biology migration 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor Gene expression Gene Knockdown Techniques Cluster Analysis Humans Radiology Nuclear Medicine and imaging esophageal cancer long noncoding RNA Original Research Cancer Biology CASC9 Aged Cell Proliferation Neoplasm Staging Gene knockdown Cell growth Gene Expression Profiling RNA High-Throughput Nucleotide Sequencing Cell migration Middle Aged invasion Molecular biology Long non-coding RNA digestive system diseases Gene Expression Regulation Neoplastic 030104 developmental biology Oncology 030220 oncology & carcinogenesis Lymphatic Metastasis Female RNA Long Noncoding |
| Zdroj: | Cancer Medicine |
| ISSN: | 2045-7634 |
| Popis: | The objective of the study was to investigate the expression and functions of CASC9 in esophageal squamous cell carcinoma (ESCC). Long noncoding RNAs (lncRNAs) upregulated in ESCC tissues were detected by RNA sequencing. Expression of CASC9 was determined from clinical samples and cell lines by qRT‐PCR. The effects of CASC9 knockdown on migration and invasion were evaluated by wound healing assay, cell migration and invasion assays in vitro. We found that the lncRNA, CASC9, was markedly upregulated in ESCC tissues. Furthermore, knockdown of CASC9 significantly suppressed cell migration and invasion in vitro. Furthermore, enhanced CASC9 expression level was correlated with differentiation. The results indicated that CASC9 is significantly upregulated in ESCC tissues and may represent a new marker of poor prognosis and a potential therapeutic target for esophageal cancer intervention. |
| Databáze: | OpenAIRE |
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