AGO1-miR173 complex initiates phased siRNA formation in plants
| Autor: | Christopher M. Sullivan, Noah Fahlgren, Seong Jeon Yoo, Sunny D. Gilbert, Miya D. Howell, Ji Hoon Ahn, Edwards Allen, Taiowa A. Montgomery, Amanda L. Alexander, Goretti Nguyen, James C. Carrington |
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| Rok vydání: | 2008 |
| Předmět: |
Genetics
Phytoene desaturase Multidisciplinary biology Arabidopsis Proteins Trans-acting siRNA RNA Biological Sciences Argonaute biology.organism_classification Cell biology MicroRNAs RNA Plant Arabidopsis Argonaute Proteins RasiRNA Pentatricopeptide repeat RNA Messenger RNA Small Interfering Biogenesis |
| Zdroj: | Proceedings of the National Academy of Sciences. 105:20055-20062 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | MicroRNA (miRNA)-guided cleavage initiates entry of primary transcripts into the transacting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6, DICER-LIKE4, and SUPPRESSOR OF GENE SILENCING3. Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173-guided initiation–cleavage of primary transcripts and target several transcripts encoding pentatricopeptide repeat proteins and proteins of unknown function. Here, the TAS1c locus was modified to produce synthetic (syn) tasiRNA to target an endogenous transcript encoding PHYTOENE DESATURASE and used to analyze the role of miR173 in routing of transcripts through the tasiRNA pathway. miR173 was unique from other miRNAs in its ability to initiate TAS1c -based syn-tasiRNA formation. A single miR173 target site was sufficient to route non- TAS transcripts into the pathway to yield phased siRNA. We also show that miR173 functions in association with ARGONAUTE 1 (AGO1) during TAS1 and TAS2 tasiRNA formation, and we provide data indicating that the miR173–AGO1 complex possesses unique functionality that many other miRNA–AGO1 complexes lack. |
| Databáze: | OpenAIRE |
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