Identification of metallo-β-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection
| Autor: | Saeed Khoshnood, Aghil Bahramian, Nader Hashemi, Morteza Saki, Melika Moradi, Mohammadmahdi Karimi-Yazdi, Nahid Jalallou |
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| Rok vydání: | 2021 |
| Předmět: |
Hemodialysis patients
Imipenem medicine.drug_class Antibiotics Microbial Sensitivity Tests Biology Fosfomycin medicine.disease_cause Meropenem Metallo-β-lactamases beta-Lactamases Microbiology law.invention Bacterial Proteins law Renal Dialysis Ampicillin Genetics medicine polycyclic compounds Escherichia coli Humans AmpC Cefoxitin Molecular Biology Polymerase chain reaction Escherichia coli Infections Escherichia coli Proteins Drug Resistance Microbial General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Urinary tract infections Anti-Bacterial Agents Carbapenems bacteria Original Article medicine.drug |
| Zdroj: | Molecular Biology Reports |
| ISSN: | 1573-4978 |
| Popis: | Background This study aimed to identify metallo-β-lactamases (MBLs) and AmpC β-lactamases-producing Escherichia coli isolates obtained from hemodialysis (HD) patients with urinary tract infections (UTI). Methods and results A total of 257 HD patients with UTI were included in this study, from which 47 E. coli isolates were collected. Antibiotic susceptibility was tested by disc diffusion method. MBLs and AmpC production were phenotypically detected by imipenem-ethylenediaminetetracetate and cefoxitin/boronic acid assays, respectively. The presence of MBLs and AmpC genes was examined by polymerase chain reaction (PCR). Fosfomycin and ampicillin were the most and the least effective antibiotics against E. coli isolates, respectively. Moreover, 61.7% (29/47) of E. coli isolates were multidrug-resistant with seven different antibiotypes. Antibiotype V (AMP–CIP–IMP–MEM–CPD–CRO–CTX–GEN–LEV–SXT–TOB) was the most prevalent profile. Besides, 24 (51.1%) isolates were simultaneously resistant to imipenem and meropenem. Phenotypic assay showed MBL production in 16 (66.7%) of the 24 carbapenem-resistant E. coli isolates. The distribution of MBL genes in carbapenem-resistant E. coli was as follows: blaIMP 18 (72%), blaVIM 7 (28%), and blaNDM 1 (4%). AmpC was detected in 61.7% (29/47) of the isolates using the phenotypic method. The presence of AmpC genes was confirmed by PCR in only 26 of 29 (86.7%) AmpC producers. The frequencies of blaDHA-1, blaACC, and blaCMY-2 were 6 (20.7%), 11 (37.9%), and 21 (72.4%), respectively. Conclusions The emergence of MBL and AmpC coproducing E. coli isolates calls for an urgent surveillance program for timely diagnosis and screening of these genes in our healthcare systems. |
| Databáze: | OpenAIRE |
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