Long term in vitro-cultured plant cells show typical neoplastic features at the cytological level
| Autor: | Jean Wüest, Thomas Gaspar, Michèle Crèvecoeur, Julien Häsler |
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| Rok vydání: | 2003 |
| Předmět: |
Time Factors
Nucleolus Plant Growth Regulators/metabolism Exogenous hormones Biology Beta vulgaris/cytology/ultrastructure Cell Line Starch/metabolism Cell wall Plant Growth Regulators Cell Wall Plant Cells Plant Tumors Neoplastic progression Plants/cytology/ultrastructure Cell Wall/metabolism Cell Nucleus Regeneration (biology) Starch Cell Biology General Medicine Anatomy Plants Plant cell In vitro Cell biology Plant Leaves Microscopy Electron ddc:580 Callus Cell Nucleus/metabolism Microscopy Electron Scanning Plant Leaves/cytology/ultrastructure Beta vulgaris |
| Zdroj: | Biology of the Cell, Vol. 95, No 6 (2003) pp. 357-64 |
| ISSN: | 0248-4900 |
| DOI: | 10.1016/s0248-4900(03)00077-7 |
| Popis: | Cells from a green normal (dependent on exogenous hormones) callus and from an achlorophyllous fully habituated (independent from exogenous hormones) callus, both generated from the same sugarbeet strain more than twenty years ago, were reexamined cytologically, ten years after the first comparative description. Cells from the habituated callus, already considered as neoplastic cells, because terminating a neoplastic progression where the organogenic totipotency was lost, still showed nuclear invaginations, polynucleolation, vacuolation of nucleoli and incomplete cell walls, nevertheless at a higher degree. The present study particularly shows that, compared to their previous description, normal cells have started to acquire some features (polynucleolation, nuclear invaginations...) that are typical of the neoplastic cells. This suggests that normal cells, on the long term, also entered a neoplastic progression, which should explain the known progressive loss of regeneration capacity of too long subcultured hormone-dependent calli. |
| Databáze: | OpenAIRE |
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