Lack of effect of hormonal treatment and smoking on cytokeratin expression in buccal mucosa
| Autor: | Risto-Pekka Happonen, Stina Syrjänen, Riitta Leimola-Virtanen, Ismo Virtanen, Marita Luomanen |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Cytoplasm Cancer Research Pathology medicine.medical_specialty medicine.drug_class medicine.medical_treatment Gene Expression Biology Monoclonal antibody Epithelium Pathology and Forensic Medicine Andrology 03 medical and health sciences Basal (phylogenetics) Cytokeratin 0302 clinical medicine Keratin medicine Humans Fluorescent Antibody Technique Indirect Progesterone Fluorescent Dyes 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chemotherapy Estradiol Staining and Labeling Smoking Mouth Mucosa Antibodies Monoclonal 3. Good health Staining Otorhinolaryngology chemistry 030220 oncology & carcinogenesis Keratins Periodontics Immunohistochemistry Female Oral Surgery Fluorescein-5-isothiocyanate Contraceptives Oral Hormone |
| Zdroj: | Journal of Oral Pathology and Medicine. 24:251-254 |
| ISSN: | 1600-0714 0904-2512 |
| DOI: | 10.1111/j.1600-0714.1995.tb01177.x |
| Popis: | Differences in cytokeratin expression of clinically normal buccal mucosa were studied in 50 healthy women by indirect immunofluorescence staining with monoclonal antibodies. The subjects were divided into four groups: control group (N = 18), smokers (N = 8), oral contraceptive users (N = 8) and smokers receiving oral contraceptives (N = 16). Our findings indicate that cytokeratin expression in noncornified stratified epithelium is not influenced by smoking or oestradiol/progesterone treatment. Only cytokeratin No. 19 showed variable patterns of expression but the differences could not be ascribed to smoking or contraceptives. Cytokeratin No. 19 gave a positive reaction in the basal and suprabasal layers in 34 subjects (68%). In 9 (18%) specimens, the staining was positive in the basal cells and showed a positive heterogeneous cytoplasmic reaction in the suprabasal cells. Interestingly, cytokeratin No. 7 was recognized in all epithelial cells except the basal cells. Our results suggest that changes in the serum oestradiol levels do not affect the cytokeratin pattern in buccal mucosa. |
| Databáze: | OpenAIRE |
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