Detection of inflammation and oxidative lung injury in exhaled breath condensate of rats with acute lung injury due to Staphylococcal enterotoxin B
| Autor: | Rekha Solomon, Sabrina M. Heidemann, Suwannee Phumeetham, Hitesh Sandhu, Keshava Murthy Narayana Gowda |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty Acute Lung Injury Inflammation Oxidative phosphorylation Enterotoxin Lung injury Gastroenterology Rats Sprague-Dawley Enterotoxins Internal medicine medicine Animals Exhaled breath condensate Leukotriene business.industry Rats respiratory tract diseases Disease Models Animal Breath Tests Exhalation Immunology Tumor necrosis factor alpha Gases medicine.symptom business Biomarkers Lung lavage |
| Zdroj: | Journal of Breath Research. 7:026003 |
| ISSN: | 1752-7163 1752-7155 |
| DOI: | 10.1088/1752-7155/7/2/026003 |
| Popis: | The objectives were to determine if tumor necrosis factor (TNF)-α, leukotriene (LT)B4 and 8-isoprostane (IP) were present in the exhaled breath condensate (EBC) and total lung lavage (TLL) of mechanically ventilated rats, 4 and 24 h after administration of Staphylococcal entertoxin (SEB) and to find out if these mediators in the EBC correlate with the concentration in the TLL. Rats were assigned to control (n = 8); 4 h (n = 8) or 24 h (n = 8) groups after SEB. The rats were mechanically ventilated and EBC and TLL were collected for TNF-α, LTB4 and 8-IP. TNF-α was higher in the EBC of rats at 24 h after SEB when compared to control [34.5 (16–62 versus 2 (14–30) pg ml−1, p < 0.05] and also in the TLL [113.5 (70–460) versus 59 (35–79) pg ml−1, p < 0.04]. LTB4 was higher in the EBC of 24 h SEB rats, when compared to control [42 (28–50) versus 36 (29–37) pg ml−1, p < 0.01] and also in the TLL [179 (116–232) versus 114 (80–187) pg ml−1, p < 0.05). 8-IP was similar among the groups. No correlation was observed between TNF-α, LTB4 or 8-IP in the EBC compared to the TLL. TNF-α and LTB4 may be indicators of inflammation and oxidative lung injury but the EBC does not correlate with TLL concentrations. |
| Databáze: | OpenAIRE |
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