Dietary broccoli mildly improves neuroinflammation in aged mice but does not reduce lipopolysaccharide-induced sickness behavior

Autor: Elizabeth H. Jeffery, Rodney W. Johnson, Yung Ju Chen, Brigitte E. Townsend
Rok vydání: 2014
Předmět:
Lipopolysaccharides
Aging
HMOX1
Endocrinology
Diabetes and Metabolism
Interleukin-1beta
medicine.disease_cause
Mice
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
BALB/c mice
Illness Behavior
2. Zero hunger
Glucoraphanin
Mice
Inbred BALB C
0303 health sciences
Nutrition and Dietetics
Behavior
Animal
Brain
food and beverages
Up-Regulation
3. Good health
Liver
Receptors
Chemokine
Microglia
medicine.symptom
medicine.medical_specialty
LPS
NF-E2-Related Factor 2
CX3C Chemokine Receptor 1
Inflammation
Brassica
Biology
Article
Proinflammatory cytokine
03 medical and health sciences
Internal medicine
Glial Fibrillary Acidic Protein
medicine
Animals
RNA
Messenger
Neuroinflammation
030304 developmental biology
Broccoli
Heme oxygenase
chemistry
Immunology
Sulforaphane
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Nutrition Research. 34(11):990-999
ISSN: 0271-5317
DOI: 10.1016/j.nutres.2014.10.001
Popis: Aging is associated with oxidative stress and heightened inflammatory response to infection. Dietary interventions to reduce these changes are therefore desirable. Broccoli contains glucoraphanin, which is converted to sulforaphane (SFN) by plant myrosinase during cooking preparation or digestion. Sulforaphane increases antioxidant enzymes including NAD(P)H quinone oxidoreductase and heme oxygenase I and inhibits inflammatory cytokines. We hypothesized that dietary broccoli would support an antioxidant response in brain and periphery of aged mice and inhibit lipopolysaccharide (LPS)–induced inflammation and sickness. Young adult and aged mice were fed control or 10% broccoli diet for 28 days before an intraperitoneal LPS injection. Social interactions were assessed 2, 4, 8, and 24 hours after LPS, and mRNA was quantified in liver and brain at 24 hours. Dietary broccoli did not ameliorate LPS-induced decrease in social interactions in young or aged mice. Interleukin-1β (IL-1β) expression was unaffected by broccoli consumption but was induced by LPS in brain and liver of adult and aged mice. In addition, IL-1β was elevated in brain of aged mice without LPS. Broccoli consumption decreased age-elevated cytochrome b-245 β, an oxidative stress marker, and reduced glial activation markers in aged mice. Collectively, these data suggest that 10% broccoli diet provides a modest reduction in age-related oxidative stress and glial reactivity, but is insufficient to inhibit LPS-induced inflammation. Thus, it is likely that SFN would need to be provided in supplement form to control the inflammatory response to LPS.
Databáze: OpenAIRE
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