Twisting of the zebrafish heart tube during cardiac looping is a tbx5-dependent and tissue-intrinsic process
| Autor: | Fabian Kruse, Federico Tessadori, Erika Tsingos, Vincent M. Christoffels, Malou van den Boogaard, Susanne C. van den Brink, Roeland M. H. Merks, Enrico Sandro Colizzi, Jeroen Bakkers |
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| Přispěvatelé: | Medical Biology, ARD - Amsterdam Reproduction and Development, ACS - Heart failure & arrhythmias, Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Science Transcription Factors/genetics Morphogenesis heart T-box General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Embryo Nonmammalian/embryology Zebrafish Proteins/genetics Animals Biology (General) Process (anatomy) Zebrafish Body Patterning 030304 developmental biology 0303 health sciences Organogenesis/genetics General Immunology and Microbiology biology Chemistry General Neuroscience Embryogenesis General Medicine biology.organism_classification Cell biology chiral cell tracking Embryo laterality Cardiac chamber cardiovascular system Medicine Atrioventricular canal Zebrafish/embryology Nonmammalian/embryology Heart/embryology asymmetry 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | eLife, 10:e61733. eLife Sciences Publications eLife, 10. ELIFE SCIENCES PUBLICATIONS LTD eLife, Vol 10 (2021) eLife, 10. eLife Sciences Publications |
| ISSN: | 2050-084X |
| Popis: | Organ laterality refers to the left-right asymmetry in disposition and conformation of internal organs and is established during embryogenesis. The heart is the first organ to display visible left-right asymmetries through its left-sided positioning and rightward looping. Here, we present a new zebrafish loss-of-function allele for tbx5a, which displays defective rightward cardiac looping morphogenesis. By mapping individual cardiomyocyte behavior during cardiac looping, we establish that ventricular and atrial cardiomyocytes rearrange in distinct directions. As a consequence, the cardiac chambers twist around the atrioventricular canal resulting in torsion of the heart tube, which is compromised in tbx5a mutants. Pharmacological treatment and ex vivo culture establishes that the cardiac twisting depends on intrinsic mechanisms and is independent from cardiac growth. Furthermore, genetic experiments indicate that looping requires proper tissue patterning. We conclude that cardiac looping involves twisting of the chambers around the atrioventricular canal, which requires correct tissue patterning by Tbx5a. |
| Databáze: | OpenAIRE |
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