Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
| Autor: | Mark L. Johnson, Hammad Mumtaz, Mark T. Begonia, JoAnna M. Scott, Nuria Lara-Castillo, Thiagarajan Ganesh, Mark Dallas |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Aging lcsh:Diseases of the musculoskeletal system Three point flexural test Endocrinology Diabetes and Metabolism Ulna 030209 endocrinology & metabolism Bending Article 03 medical and health sciences 0302 clinical medicine Age related Medicine Orthopedics and Sports Medicine Femur Tibia Three-point bending Orthodontics business.industry Gender C57BL/6N Biomechanical parameters medicine.anatomical_structure Cortical bone 030101 anatomy & morphology lcsh:RC925-935 business Femur tibia |
| Zdroj: | Bone Reports Bone Reports, Vol 12, Iss, Pp 100266-(2020) |
| ISSN: | 2352-1872 |
| Popis: | Aging is known to reduce bone quality and bone strength. We sought to determine how aging affects the biomechanical and architectural properties of various long bones, and if sex influences age related differences/changes. While researchers have extensively studied these changes in individual bones of mice, there is no comprehensive study of the changes in the bones from the same mice to study the changes with aging. We performed three point bending tests and microcomputed tomography (microCT) analysis on femurs, tibiae and ulnae. Three point bending tests were utilized to calculate biomechanical parameters and imaging was also performed using high resolution microCT to reveal both cortical and trabecular microarchitecture C57BL/6N mice were divided into three age groups: 6, 12 and 22 months. Each age and sex group consisted of 6–7 mice. The ultimate load to failure (UL), elastic stiffness (ES), modulus of elasticity (E) and the moment of inertia about bending axis (MOI) for each bone was calculated using three point bending test. MicroCT scans of all the bones were analyzed to determine cortical bone volume per tissue volume (C.BV/TV), trabecular bone volume per tissue volume (Tb.BV/TV), cortical bone area (B.Ar) using CTAn's microCT analysis and tested for correlation with the biomechanical parameters. Mean (standard error) values of UL in femur decreased from 19.8(0.6) N to 12.8(1.1) N (p Highlights • Assess age/gender related biomechanical property changes in femur, tibiae and ulnae. • C57Bl/6 mice were divided into three age groups: 6, 12 and 22 months. • Significant decline in the biomechanical response of femurs and tibiae at the age of 22 months. • Ulnae showed some improved properties with aging. • Aging affected biomechanical rather than microCT properties and female bones |
| Databáze: | OpenAIRE |
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