Measurement of protein-ligand binding constants from reaction-diffusion concentration profiles

Autor:	Bartosz A. Grzybowski, Paul J. Wesson, Yanhu Wei, Igor V. Kourkine
Rok vydání:	2010
Předmět:	Chemistry Diffusion Analytical chemistry Proteins Ligand (biochemistry) Ligands Analytical Chemistry Dissociation constant Kinetics Protein structure Reaction–diffusion system Self-healing hydrogels Biophysics Naked eye Gels Protein ligand Protein Binding
Zdroj:	Analytical chemistry. 82(21)
ISSN:	1520-6882
Popis:	Protein-ligand dissociation constants, K(d), are determined precisely and down to the picomolar range from reaction-diffusion (RD) concentration profiles created by proteins diffusing through hydrogels functionalized with protein ligands. The RD process effectively amplifies the molecular-scale binding events into macroscopic patterns visible to the naked eye. The method is applicable to various protein-ligand pairs and does not require any prior knowledge about the protein structure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8229586f1ed2472ec1813377fa912b1 https://pubmed.ncbi.nlm.nih.gov/20923152 Zobrazit plný text záznamu