Determination of candesartan cilexetil, candesartan and a metabolite in human plasma and urine by liquid chromatography and fluorometric detection
| Autor: | Cathrine Andersen, Per-Olof Lagerström, Helene Stenhoff |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Detection limit
Chromatography Chemistry Metabolite Biphenyl Compounds Reproducibility of Results Tetrazoles Angiotensin II receptor antagonist General Chemistry Urine Prodrug Sensitivity and Specificity Angiotensin II Candesartan chemistry.chemical_compound Spectrometry Fluorescence medicine Humans Benzimidazoles Prodrugs Quantitative analysis (chemistry) Antihypertensive Agents Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 731:411-417 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(99)00247-9 |
| Popis: | Liquid chromatographic methods are described for the determination of a new effective anti-hypertensive drug candesartan (CV-11974), its prodrug candesartan cilexetil (TCV-116) and a metabolite, CV-15959 in human plasma and urine. The assays comprise liquid-liquid extraction and separation on a phenyl column with fluorometric detection. The methods give absolute recoveries of 70, 83 and 78% for candesartan cilexetil, candesartan and CV-15959, respectively, and the limit of quantification is 5, 1 and 3 nM of plasma (RSD < 20%), respectively. The methods were applied to plasma and urine samples from biopharmaceutical and clinical studies in man. |
| Databáze: | OpenAIRE |
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