A delta 4-3-keto pathway for testosterone synthesis in the human spermatozoa
Autor: | Annamalai Loganath, Shan S. Ratnam, P. C. T. Chew, R. Gunasegaram, K. L. Peh |
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Rok vydání: | 1998 |
Předmět: |
Adult
Male medicine.medical_specialty Radioisotope Dilution Technique medicine.drug_class Semen Biology In Vitro Techniques Male infertility Endocrinology Internal medicine medicine Humans Testosterone Infertility Male Progesterone chemistry.chemical_classification Sperm Count Metabolism Middle Aged Androgen medicine.disease Sperm Spermatozoa Enzyme chemistry Pregnenolone Chromatography Thin Layer medicine.drug |
Zdroj: | Archives of andrology. 40(1) |
ISSN: | 0148-5016 |
Popis: | The ability of human spermatozoa to metabolize pregnenolone to progesterone and progesterone to testosterone was assessed. Sonicated specimens of freshly ejaculated sperm from two groups of husbands (n = 6, age 32-38 years; n = 6, age 30-51 years) of infertile couples in the range of sperm concentration between 237.5 and 568.5, 100.1 and 248.8 millions per ejaculate, were separately incubated with [7n-3H]pregnenolone and [1,2,6,7,16,17-3H]progesterone. Using the classical reverse-isotope dilution technique the desired products [3H]progesterone and [3H]testosterone formed from the respective substrates were isolated and characterized, yielding 1.4 to 12.2% and 3.1 x 10(-2) to 2.0 x 10(-1)%. Such metabolites were not evident in the controls. The results indicate that the human spermatozoa contain the enzymes necessary for the transformation of pregnenolone to testosterone via the delta 4-3-keto route. |
Databáze: | OpenAIRE |
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