Colon-derived liver metastasis, colorectal carcinoma, and hepatocellular carcinoma can be discriminated by the Ca(2+)-binding proteins S100A6 and S100A11
Autor: | Bettina Schimmel, Annett Bleul, Christian Melle, Ferdinand von Eggeling, Günther Ernst |
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Jazyk: | angličtina |
Rok vydání: | 2008 |
Předmět: |
Oncology
medicine.medical_specialty Pathology/Histopathology Carcinoma Hepatocellular Gastroenterology and Hepatology/Gastrointestinal Cancers Proteome Colorectal cancer Colon lcsh:Medicine Cell Cycle Proteins Oncology/Gastrointestinal Cancers Biology Proteomics Metastasis S100 Calcium Binding Protein A6 Internal medicine Calcium-binding protein Cell Line Tumor Carcinoma medicine Humans lcsh:Science Multidisciplinary Calcium-Binding Proteins Liver Neoplasms S100 Proteins lcsh:R medicine.disease Primary tumor Immunohistochemistry digestive system diseases Hepatocellular carcinoma Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization lcsh:Q Colorectal Neoplasms Research Article |
Zdroj: | PLoS ONE, Vol 3, Iss 12, p e3767 (2008) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Background It is unknown, on the proteomic level, whether the protein patterns of tumors change during metastasis or whether markers are present that allow metastases to be allocated to a specific tumor entity. The latter is of clinical interest if the primary tumor is not known. Methodology/Principal Findings In this study, tissue from colon-derived liver metastases (n = 17) were classified, laser-microdissected, and analysed by ProteinChip arrays (SELDI). The resulting spectra were compared with data for primary colorectal (CRC) and hepatocellular carcinomas (HCC) from our former studies. Of 49 signals differentially expressed in primary HCC, primary CRC, and liver metastases, two were identified by immunodepletion as S100A6 and S100A11. Both proteins were precisely localized immunohistochemically in cells. S100A6 and S100A11 can discriminate significantly between the two primary tumor entities, CRC and HCC, whereas S100A6 allows the discrimination of metastases and HCC. Conclusions Both identified proteins can be used to discriminate different tumor entities. Specific markers or proteomic patterns for the metastases of different primary cancers will allow us to determine the biological characteristics of metastasis in general. It is unknown how the protein patterns of tumors change during metastasis or whether markers are present that allow metastases to be allocated to a specific tumor entity. The latter is of clinical interest if the primary tumor is not known. |
Databáze: | OpenAIRE |
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