E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor
| Autor: | Robert J. Linhardt, Jonathan S. Dordick, Anne Marie Hickey, Fuming Zhang, Amy Suen, Zhenyu Wang, Mellisa Ly, Weihong Zhong |
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| Rok vydání: | 2010 |
| Předmět: |
Bioengineering
medicine.disease_cause Polysaccharide Applied Microbiology and Biotechnology Article Escherichia coli medicine Polyacrylamide gel electrophoresis Glycosaminoglycans chemistry.chemical_classification Chromatography Molecular Structure biology Heparin Polysaccharides Bacterial biology.organism_classification Enterobacteriaceae Molecular Weight Chemically defined medium Biochemistry chemistry Yield (chemistry) Fermentation Bacteria Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 107:964-973 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.22898 |
| Popis: | Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioen- gineered heparin. Heparosan is also the capsular polysac- charide of Escherichia coli K5 strain. The current study was focused on the examination of the fermentation of E. coli K5 with the goal of producing heparosan in high yield and volumetric productivity. The structure and molecular weight properties of this bacterial heparosan were deter- mined using polyacrylamide gel electrophoresis (PAGE) and Fourier transform mass spectrometry. Fermentation of E. coli K5 in a defined medium using exponential fed-batch glucose addition with oxygen enrichment afforded hepar- osan at 15g/L having a number average molecular weight of 58,000Da and a weight average molecular weight of 84,000Da. Biotechnol. Bioeng. 2010;107: 964-973. |
| Databáze: | OpenAIRE |
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