Mixed infections and rifampin hetero-resistance of Mycobacterium tuberculosis clinical isolates
| Autor: | Qun Sun, Mei Luo, Ke Tang, Yuqing Li, David Couvin, Honghu Sun, Zhongyue Luo, Song Li, Nalin Rastogi, Qingxia He, Rui Pi, Chao Zheng |
|---|---|
| Přispěvatelé: | Key Laboratory of Bio-resources and Eco-environment, ministry of education-College of Life Sciences-Sichuan University [Chengdu] (SCU), Chengdu Medical College, Chengdu, Sichuan, P. R. China, Chengdu Public Health Clinical Center, Chengdu, Sichuan, P. R. China, Sichuan University [Chengdu] (SCU), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), financial support by the National Nature Science Foundation of China (31200985). |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Mixed infections Epidemiology Antitubercular Agents Hetero-resistance Genotype Child Aged 80 and over Spoligotyping 0303 health sciences Coinfection Genotypic lineages DNA-Directed RNA Polymerases Middle Aged 3. Good health Child Preschool Female Rifampin Mixed infection Adult Microbiology (medical) China Tuberculosis Adolescent MIRU-VNTRs Biology Microbiology Mycobacterium tuberculosis Young Adult 03 medical and health sciences Tandem repeat Drug Resistance Bacterial medicine Humans Aged Retrospective Studies 030304 developmental biology RpoB 030306 microbiology Miru vntrs Sequence Analysis DNA Odds ratio biology.organism_classification rpoB medicine.disease Virology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Molecular Typing Cross-Sectional Studies |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2015, 53 (7), pp.2138-2147. ⟨10.1128/JCM.03507-14⟩ |
| ISSN: | 0095-1137 |
| DOI: | 10.1128/JCM.03507-14⟩ |
| Popis: | Mixed infections and heteroresistance of Mycobacterium tuberculosis contribute to the difficulty of diagnosis, treatment, and control of tuberculosis. However, there is still no proper solution for these issues. This study aimed to investigate the potential relationship between mixed infections and heteroresistance and to determine the high-risk groups related to these factors. A total of 499 resistant and susceptible isolates were subjected to spoligotyping and 24-locus variable-number tandem repeat methods to analyze their genotypic lineages and the occurrence of mixed infections. Two hundred ninety-two randomly selected isolates were sequenced on their rpoB gene to examine mutations and heteroresistance. The results showed that 12 patients had mixed infections, and the corresponding isolates belonged to Manu2 ( n = 8), Beijing ( n = 2), T ( n = 1), and unknown ( n = 1) lineages. Manu2 was found to be significantly associated with mixed infections (odds ratio, 47.72; confidence interval, 9.68 to 235.23; P < 0.01). Four isolates (1.37%) were confirmed to be heteroresistant, which was caused by mixed infections in three (75%) isolates; these belonged to Manu2. Additionally, 3.8% of the rifampin-resistant isolates showing no mutation in the rpoB gene were significantly associated with mixed infections (χ 2 , 56.78; P < 0.01). This study revealed for the first time that Manu2 was the predominant group in the cases of mixed infections, and this might be the main reason for heteroresistance and a possible mechanism for isolates without any mutation in the rpoB gene to become rifampin resistant. Further studies should focus on this lineage to clarify its relevance to mixed infections. |
| Databáze: | OpenAIRE |
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