The small viral membrane-associated protein P32 is involved in bacteriophage PRD1 DNA entry
| Autor: | A. Marika Grahn, Dennis H. Bamford, Rimantas Daugelavičius |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Phage display
Genes Viral Phagemid viruses Immunology Molecular Sequence Data Biology Virus Replication Microbiology Permeability 03 medical and health sciences chemistry.chemical_compound Viral Envelope Proteins Viral entry Cell Wall Virology Gram-Negative Bacteria Bacteriophage PRD1 Amino Acid Sequence 030304 developmental biology 0303 health sciences 030306 microbiology Viral membrane Molecular biology Cell biology Virus-Cell Interactions Molecular Weight chemistry Lytic cycle Insect Science Host cell cytoplasm Host cell envelope DNA Gene Deletion |
| Zdroj: | Journal of virology. 76(10) |
| ISSN: | 0022-538X |
| Popis: | The lipid-containing bacteriophage PRD1 infects a variety of gram-negative cells by injecting its linear double-stranded DNA genome into the host cell cytoplasm, while the protein capsid is left outside. The virus membrane and several structural proteins are involved in phage DNA entry. In this work we identified a new infectivity protein of PRD1. Disruption of geneXXXIIresulted in a mutant phenotype defective in phage reproduction. The absence of the protein P32 did not compromise the particle assembly but led to a defect in phage DNA injection. In P32-deficient particles the phage membrane is unable to undergo a structural transformation from a spherical to a tubular form. Since P32−particles are able to increase the permeability of the host cell envelope to a degree comparable to that found with wild-type particles, we suggest that the tail-tube formation is needed to eject the DNA from the phage particle rather than to reach the host cell interior. |
| Databáze: | OpenAIRE |
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