MORPHOLOGY AND ATP-ASE OF ISOLATED MITOCHONDRIA
| Autor: | Michael L. Watson, Robert F. Witter, Mary A. Cottone |
|---|---|
| Rok vydání: | 1955 |
| Předmět: |
chemistry.chemical_classification
animal structures Sucrose Phosphatase Mitochondria Liver Cell Biology Mitochondrion Biology Article Phosphoric Monoester Hydrolases Mitochondria Rats law.invention chemistry.chemical_compound Adenosine Triphosphate Enzyme Liver chemistry Biochemistry Mitochondrial matrix law Animals Electron microscope Citric acid Adenosine triphosphate |
| Zdroj: | The Journal of Biophysical and Biochemical Cytology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.1.2.127 |
| Popis: | Changes in the morphology of rat liver mitochondria brought about by different methods of isolation and the concomitant changes in ATP-ase activity were studied. The morphology was investigated with the electron microscope. It was found that the ATP-ase activity of the isolated mitochondria cannot be readily correlated with the morphology of the mitochondria. The ATP-ase found in these preparations was latent, resembling the enzyme described in mitochondria prepared in 0.25 M sucrose. In confirmation of earlier results the use of 0.88 M sucrose yielded preparations with a higher initial ATP-ase than did other methods. Preparation in 0.25 M sucrose resulted in round, swollen mitochondria of which 30 to 40 per cent appeared to have lost a substantial part of the mitochondrial matrix. Preparations in 0.44 to 0.88 M sucrose contained mainly rod-shaped mitochondria plus a small amount of another type of swollen mitochondria. The matrix of mitochondria isolated in 0.88 M sucrose was highly condensed. By the use of 0.44 M sucrose adjusted to pH 6.2 with citric acid, it was possible to isolate, for the first time, mitochondria closely resembling those in situ and containing latent ATP-ase. |
| Databáze: | OpenAIRE |
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