| Autor: |
Jae Hwan, Shin, Tae Jung, Park, Moon Seop, Hyun, Jong Pil, Park |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Food Chemistry. 378:132061 |
| ISSN: |
0308-8146 |
| DOI: |
10.1016/j.foodchem.2022.132061 |
| Popis: |
Whole peptide-displayed phage particles are promising alternatives to antibodies in sensor development; however, greater control and functionalization of these particles are required. In this study, we aimed to identify and create highly sensitive and selective phage-based electrochemical biosensors for detecting ovomucoid, a known food allergen. Phage display was performed using two different phage libraries (cyclic and linear form of peptides), which displayed affinity peptides capable of binding specifically to ovomucoid. Throughout the biopanning, two phage clones that displayed both peptides (CTDKASSSC and WWQPYSSAPRWL) were selected. After the characterization of their binding affinities, both whole phage particles were covalently attached to a gold electrode using crosslinking chemistry (MUA-EDC/NHS and Sulfo-LC/SPDP); the developed phage sensor was characterized using cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS). The cyclic peptide-displayed phage sensor modified using EDC/NHS chemistry exhibited significantly better binding affinity (K |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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