Long-term in vitro cultivation of Plasmodium berghei

Autor:	Vijayalaxmi R. Kamath, Mira Ramaiya, D.M. Renapurkar
Rok vydání:	1987
Předmět:	Erythrocytes biology Plasmodium berghei Petri dish Temperature biology.organism_classification In vitro Culture Media law.invention Microbiology Mice Infectious Diseases law Animals Bactopeptone Parasitology Food science Optimal growth
Zdroj:	International Journal for Parasitology. 17:1329-1331
ISSN:	0020-7519
DOI:	10.1016/0020-7519(87)90099-3
Popis:	Long-term in vitro culture of Plasmodium berghei was established using the Petri dish candle jar method of Trager and Jensen (1976). Cultures were established at 22, 27 and 37°C. As optimal growth was observed at 27°C, subsequent cultivation was carried out at this temperature. RPMI 1640 medium was modified by incorporating additional glucose (1 mg ml −1 ) and bactopeptone (1 mg ml −1 ) in the medium. This medium was found suitable for maintenance of mouse erythrocytes in vitro. P. berghei cultures were maintained using candle jars and this modified RPMI 1640 medium for 45 weeks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e80edd98f3344a2cba11a2de629947df https://doi.org/10.1016/0020-7519(87)90099-3 Zobrazit plný text záznamu