Towards sustainable bioplastic production using the photoautotrophic bacterium Rhodopseudomonas palustris TIE-1
| Autor: | Rajesh Singh, Michael S. Guzman, Karthikeyan Rengasamy, Arpita Bose, Tahina Onina Ranaivoarisoa |
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| Rok vydání: | 2018 |
| Předmět: |
Nitrogen
Polymers PHB Bioengineering Electrons Applied Microbiology and Biotechnology Bioplastic Ferrous Polyhydroxybutyrate 03 medical and health sciences chemistry.chemical_compound Food science Electrodes 030304 developmental biology 0303 health sciences Bioenergy/Biofuels/Biochemicals - Review biology 030306 microbiology Chemistry Photoferroautotrophy biology.organism_classification Bioproduction Rhodopseudomonas Photoelectroautotrophy 13. Climate action Carbon dioxide Ammonium chloride Rhodopseudomonas palustris TIE-1 Rhodopseudomonas palustris Bacteria Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology |
| ISSN: | 1476-5535 |
| Popis: | Bacterial synthesis of polyhydroxybutyrates (PHBs) is a potential approach for producing biodegradable plastics. This study assessed the ability of Rhodopseudomonas palustris TIE-1 to produce PHBs under various conditions. We focused on photoautotrophy using a poised electrode (photoelectroautotrophy) or ferrous iron (photoferroautotrophy) as electron donors. Growth conditions were tested with either ammonium chloride or dinitrogen gas as the nitrogen source. Although TIE-1’s capacity to produce PHBs varied fairly under different conditions, photoelectroautotrophy and photoferroautotrophy showed the highest PHB electron yield and the highest specific PHB productivity, respectively. Gene expression analysis showed that there was no differential expression in PHB biosynthesis genes. This suggests that the variations in PHB accumulation might be post-transcriptionally regulated. This is the first study to systematically quantify the amount of PHB produced by a microbe via photoelectroautotrophy and photoferroautotrophy. This work could lead to sustainable bioproduction using abundant resources such as light, electricity, iron, and carbon dioxide. Electronic supplementary material The online version of this article (10.1007/s10295-019-02165-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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